PXD041610

PXD041610 is an original dataset announced via ProteomeXchange.

Title	Proximity labeling in Arabidopsis seedlings expressing R-degron-Turbo vs. M-degron-Turbo vs. L-degron-Turbo untreated (DMSO control), treated with 10µM Bortezomib and treated with 1µM Concanamycin A.
Description	Goal was to identify N-recognins for Arginine and Leucine by comparison of biotinylated proteins from 1 week old whole Arabidopsis seedlings constitutively expressing the bait constructs R-degron-Turbo or L-degron-Turbo. M-degron-Turbo served as a negative control. The plants were treated with 50µM biotin and either the proteasome inhibitor Bortezomib (10µM) or the inhibitor of vacuolar acidification Concanamycin A (1µM) or DMSO one hour before harvest.
HostingRepository	PRIDE
AnnounceDate	2024-04-15
AnnouncementXML	Submission_2024-04-15_04:46:19.218.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	WeiQiang Chen
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	biotinylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-17 23:41:26	ID requested
⏵ 1	2024-04-15 04:46:19	announced

10.1093/PLCELL/KOAE117;

submitter keyword: Bortezomib,N-degron pathway, proximity labeling, Concanamycin A, protein turnover, Turbo

Andreas Bachmair
contact affiliation	Max Perutz Labs, University of Vienna
contact email	andreas.bachmair@univie.ac.at
lab head
WeiQiang Chen
contact affiliation	Mass Spectrometry Facility, Max Perutz Laboratories Support GmbH
contact email	chenweiqiang@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/04/PXD041610

PRIDE project URI

• PRIDE
  1. PXD041610
     1. Label: PRIDE project
     2. Name: Proximity labeling in Arabidopsis seedlings expressing R-degron-Turbo vs. M-degron-Turbo vs. L-degron-Turbo untreated (DMSO control), treated with 10µM Bortezomib and treated with 1µM Concanamycin A.