PXD040974 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Metabolic labelling and LC-MS/MS-based identification of IL-1α-induced secreted proteomes from epithelial cells in the presence or absence of serum |
Description | The unbiased identification of cytokine-induced, secreted proteins from cells cultured in serum-containing medium is challenging. Here, we describe an experimental and bioinformatics workflow to label IL-1α-regulated proteins in living cells with the methionine analogue L-homopropargylglycine (L-HPG), purify and identify them by means of a CLICK-chemistry based biotinylation step followed by nanoHPLC-MS/MS. A side-by-side comparison of enriched proteins and their ontologies to serum-free conditions demonstrates the sensitivity and specificity of this approach to study the inducible secreted proteomes of epithelial cells. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_09:05:39.878.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Axel Weber |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | timsTOF Pro |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-03-20 03:35:02 | ID requested | |
1 | 2023-03-21 06:48:36 | announced | |
2 | 2023-10-02 07:33:41 | announced | 2023-10-02: Updated project metadata. |
⏵ 3 | 2023-11-14 09:05:40 | announced | 2023-11-14: Updated project metadata. |
Publication List
Priester J, Meier-Soelch J, Weiser H, Heylmann D, Weber A, Linne U, Kracht M, -induced secreted proteomes from epithelial cells in the presence or absence of serum. STAR Protoc, 4(2):102195(2023) [pubmed] |
Keyword List
submitter keyword: LC-MSMS, affinity proteomics, metabolic labeling, IL-1 alpha,HPG-Labeling, secretome |
Contact List
Michael Kracht |
contact affiliation | Rudolf Buchheim Institute of Pharmacology, Justus Liebig University, Giessen, D-35392, Germany |
contact email | michael.kracht@pharma.med.uni-giessen.de |
lab head | |
Axel Weber |
contact affiliation | Rudolf-Buchheim-Institute of Pharmacology Justus Liebig University Gießen |
contact email | axel.weber@pharma.med.uni-giessen.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD040974
- Label: PRIDE project
- Name: Metabolic labelling and LC-MS/MS-based identification of IL-1α-induced secreted proteomes from epithelial cells in the presence or absence of serum