PXD040974

PXD040974 is an original dataset announced via ProteomeXchange.

Title	Metabolic labelling and LC-MS/MS-based identification of IL-1α-induced secreted proteomes from epithelial cells in the presence or absence of serum
Description	The unbiased identification of cytokine-induced, secreted proteins from cells cultured in serum-containing medium is challenging. Here, we describe an experimental and bioinformatics workflow to label IL-1α-regulated proteins in living cells with the methionine analogue L-homopropargylglycine (L-HPG), purify and identify them by means of a CLICK-chemistry based biotinylation step followed by nanoHPLC-MS/MS. A side-by-side comparison of enriched proteins and their ontologies to serum-free conditions demonstrates the sensitivity and specificity of this approach to study the inducible secreted proteomes of epithelial cells.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:05:39.878.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Axel Weber
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-20 03:35:02	ID requested
1	2023-03-21 06:48:36	announced
2	2023-10-02 07:33:41	announced	2023-10-02: Updated project metadata.
⏵ 3	2023-11-14 09:05:40	announced	2023-11-14: Updated project metadata.

Priester J, Meier-Soelch J, Weiser H, Heylmann D, Weber A, Linne U, Kracht M, -induced secreted proteomes from epithelial cells in the presence or absence of serum. STAR Protoc, 4(2):102195(2023) [pubmed]

submitter keyword: LC-MSMS, affinity proteomics, metabolic labeling, IL-1 alpha,HPG-Labeling, secretome

Michael Kracht
contact affiliation	Rudolf Buchheim Institute of Pharmacology, Justus Liebig University, Giessen, D-35392, Germany
contact email	michael.kracht@pharma.med.uni-giessen.de
lab head
Axel Weber
contact affiliation	Rudolf-Buchheim-Institute of Pharmacology Justus Liebig University Gießen
contact email	axel.weber@pharma.med.uni-giessen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD040974

PRIDE project URI

• PRIDE
  1. PXD040974
     1. Label: PRIDE project
     2. Name: Metabolic labelling and LC-MS/MS-based identification of IL-1α-induced secreted proteomes from epithelial cells in the presence or absence of serum