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PXD040716-1

PXD040716 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe GlycoPaSER prototype as a real-time N-glycopeptide identification tool based on the PaSER parallel computing platform
DescriptionReal-time database searching allows for simpler and automated proteomics workflows as it eliminates technical bottlenecks in high throughput experiments. Most importantly, it enables results dependent acquisition (RDA) where search results can be used to guide data acquisition during acquisition. This is especially beneficial for glycoproteomics since the wide range of physicochemical properties of glycopeptides lead to a wide range of optimal acquisition parameters. We established here the GlycoPaSER prototype by extending the Parallel Search Engine in Real-time (PaSER) functionality for real-time glycopeptide identification from fragmentation spectra. Glycopeptide fragmentation spectra were decomposed into peptide- and glycan-moiety spectra using common N-glycan fragments. Each moiety was subsequently identified by a specialized algorithm running in real-time. GlycoPaSER can keep up with the rate of data acquisition for real-time analysis with similar performance to other glycoproteomics software and produces results that are in line with literature reference data. The GlycoPaSER prototype presented here provides the first proof-of-concept for real-time glycopeptide identification that unlocks future development of RDA technology to transcend data acquisition.
HostingRepositoryPRIDE
AnnounceDate2023-03-13
AnnouncementXMLSubmission_2023-03-13_03:37:44.704.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterGadArmony
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumenttimsTOF Pro 2
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-03-09 02:55:13ID requested
12023-03-13 03:37:45announced
22023-11-14 09:00:37announced2023-11-14: Updated project metadata.
Publication List
Keyword List
submitter keyword: PaSER,glycoproteomics, GlycoPaSER, results dependent acquisition (RDA), real-time search
Contact List
Hans J.C.T.Wessels
contact affiliationTranslational Metabolic Laboratory, Department of Laboratory Medicine, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, 6525 GA Nijmegen, The Netherlands
contact emailHans.Wessels@radboudumc.nl
lab head
GadArmony
contact affiliationUtrecht University
contact emailgadi.armony@gmail.com
dataset submitter
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Dataset FTP location
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