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PXD040652-2

PXD040652 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic Analysis of Neural Stem Cell to Oligodendrocyte Precursor Cell Differentiation Reveals Phosphorylation-Dependent Dclk1 Processing
DescriptionThe differentiation from neural stem cells (NSCs) to oligodendrocyte precursor cells (OPCs) is only incompletely understood. In the current study, we used the so-called neurosphere assay to study this process. We performed in vitro differentiation from neurospheres (which predominantly consist of NSCs) to oligospheres (consisting mainly of OPCs) and investigated their proteome by 6plex TMT-labeling and their phosphoproteome by 3plex dimethyl labeling. Among others, we identified a continuous upregulation of double cortin like kinase 1 (Dclk1) as well as its phosphorylation sites in the so-called SP-rich region. Using western blotting and qPCR, we show that two different Dclk1 isoforms (long and short) are present in NSCs and OPCs which gradually interchange during the investigated differentiation process. We further demonstrate that Dclk1 long is proteolytically processed into Dclk1 short and that this process is regulated via phosphorylation in the SP-rich region. Finally, we generated different BioID constructs fused to individual Dclk1 domains and investigate their interactome as well as potential substrates of Dclk1.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_07:06:26.037.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRobert Hardt
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; S-carboxamidoethyl-L-cysteine; biotinylated residue; phosphorylated residue; monohydroxylated residue
InstrumentOrbitrap Fusion Lumos; LTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-03-07 03:32:53ID requested
12023-08-18 15:52:57announced
22023-11-14 07:06:26announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Phosphoproteomics, neural stem cells, dimethyl, TMT, Dclk1, neural development, proximity biotinylation, oligodendrocyte precursor cells, SILAC, differentiation,Isobaric labeling, PurA
Contact List
Dominic Winter
contact affiliationInstitute for Biochemistry and Molecular Biology, Faculty of Medicine, University of Bonn
contact emaildominic.winter@uni-bonn.de
lab head
Robert Hardt
contact affiliationInstitute for Biochemistry and Molecular Biology, Medical Faculty, University of Bonn, 53115 Bonn, Germany
contact emailrhardt@uni-bonn.de
dataset submitter
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Dataset FTP location
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