PXD040462 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Metabolic pathways operating in deteriorating versus germination-competent cyanide-primed walnut kernels |
Description | Incubation of physiologically dormant walnut kernels at warm and moist conditions leads to reduced germination and lower viability associated with triacylglycerol depletion, lipase activation and fatty acid accumulation. Cyanide however, releases kernel dormancy and improves germination accompanied with enhanced gluconeogenesis. The hypothesis that the viability loss in aging kernels is mainly due to lipid catabolism-induced oxidative stress and higher respiration was tested in a proteomic study. We used kernels with 15% moisture content aged by controlled deterioration, physiologically dormant non-aged and cyanide-treated germination primed; having low, medium and high germination potentials, respectively. We revealed 155 differentially abundant proteins out of 930 identified ones compared to dry (6% moisture content) kernels. Proteins accumulated in deteriorated kernels mainly belonged to protein folding, translation and degradation, defense, stress response and detoxification, glycolysis and fermentation but less abundant ones were related to gluconeogenesis and amino acid metabolism. Contrastingly, accumulated proteins belonged to gluconeogenesis and oxidative pentose phosphate pathway in germination-primed kernels. Deteriorated kernels also showed increased levels of H2O2, lipid peroxidation, free fatty acids, protein carbonylation, and amino acids, particularly proline but, a lower amount of sucrose and enhanced lipase, catalase, peroxidase and glutamate dehydrogenase activities. Of note, we revealed accumulation of various chaperons and proteins related to detoxification in deteriorated kernels, unlike reports on other aging seeds. Suppressed amino acid metabolism and gluconeogenesis enhanced respiration and oxidative stress in deteriorated kernels, while optimized carbon/energy metabolism promoted germination of primed kernels. |
HostingRepository | PRIDE |
AnnounceDate | 2024-02-23 |
AnnouncementXML | Submission_2024-02-23_06:39:45.752.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD040462 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Maksym Danchenko |
SpeciesList | scientific name: Juglans regia (English walnut); NCBI TaxID: 51240; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-02-27 06:17:38 | ID requested | |
⏵ 1 | 2024-02-23 06:39:46 | announced | |
Publication List
Keyword List
submitter keyword: gluconeogenesis, seed aging, Juglans regia, germination stimulation, oxidative stress, membrane lipids,proteome |
Contact List
Peter BarĂ¡th |
contact affiliation | Department of Glycobiology, Institute of Chemistry, Slovak Academy of Sciences |
contact email | peter.barath@savba.sk |
lab head | |
Maksym Danchenko |
contact affiliation | Plant Science and Biodiversity Center SAS |
contact email | maxymdan@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD040462 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD040462
- Label: PRIDE project
- Name: Metabolic pathways operating in deteriorating versus germination-competent cyanide-primed walnut kernels