PXD040285 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Uptake of oomycete RXLR effectors into host cells by clathrin-mediated endocytosis |
Description | Filamentous (oomycete and fungal) plant pathogens can deliver cytoplasmic effector proteins into host cells to facilitate disease. How RXLR effectors from the potato late blight pathogen Phytophthora infestans enter host cells is unknown. One possible route involves clathrin-mediated endocytosis (CME). Transient silencing of NbCHC, encoding clathrin heavy chain, or endosome marker NbAra6 in the model host Nicotiana benthamiana, attenuated P. infestans infection and reduced translocation of RXLR effector fusions from transgenic pathogen lines into host cells. In contrast, silencing PP1c isoforms, susceptibility factors that are not required for endocytosis, reduced infection but did not attenuate RXLR effector uptake. Endosome enrichment by ultracentrifugation and sucrose gradient fractionation revealed co-localisation of RXLR effector Pi04314-RFP with clathrin-coated vesicles. Immunopurification of clathrin- and NbAra6-associated vesicles during infection revealed that RXLR effectors Pi04314-RFP and AvrBlb1-RFP, but not apoplastic effector PiSCR74-RFP, were co-immunoprecipitated during infection with pathogen lines secreting these effectors. MS/MS analyses of proteins co-immunoprecipitated with NbAra6-GFP during infection revealed enrichment of host proteins associated with endocytic vesicles alongside multiple pathogen RXLR effectors, but not apoplastic effectors, including PiSCR74, which do not enter host cells. Our data show that uptake of P. infestans RXLR effectors into plant cells occurs via CME. |
HostingRepository | PRIDE |
AnnounceDate | 2023-07-20 |
AnnouncementXML | Submission_2023-07-20_12:15:40.881.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | HaixiaWang |
SpeciesList | scientific name: Phytophthora infestans T30-4; NCBI TaxID: 403677; scientific name: Nicotiana benthamiana; NCBI TaxID: 4100; |
ModificationList | acetylated residue; monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-02-20 07:13:11 | ID requested | |
⏵ 1 | 2023-07-20 12:16:44 | announced | |
2 | 2023-11-14 09:03:49 | announced | 2023-11-14: Updated project metadata. |
Publication List
Wang H, Wang S, Wang W, Xu L, Welsh LRJ, Gierlinski M, Whisson SC, Hemsley PA, Boevink PC, Birch PRJ, Uptake of oomycete RXLR effectors into host cells by clathrin-mediated endocytosis. Plant Cell, 35(7):2504-2526(2023) [pubmed] |
Keyword List
submitter keyword: oomycete |
RXLR effectors |
clathrin mediated endocytosis |
Contact List
PaulBirch |
contact affiliation | University of Dundee |
contact email | P.Birch@dundee.ac.uk |
lab head | |
HaixiaWang |
contact affiliation | University of Dundee |
contact email | hzjwang@dundee.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD040285
- Label: PRIDE project
- Name: Uptake of oomycete RXLR effectors into host cells by clathrin-mediated endocytosis