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PXD040207-1

PXD040207 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCell type-specific control of secondary cell wall formation by Musashi-type translational regulators in Arabidopsis
DescriptionDeciphering the mechanism of secondary cell wall/SCW formation in vascular plants is key to understanding their development and the molecular basis of biomass recalcitrance. A sophisticated network of transcription factors has been implicated in SCW synthesis in plants, but little is known about the implication of RNA-binding proteins in this process. Here we report that two RNA-binding proteins homologous to the animal translational regulator Musashi, Musashi-Like2/MSIL2 and Musashi-Like4/MSIL4, function redundantly to control SCW formation in interfascicular fibers and the setting of biomass recalcitrance. We show that the disruption of MSIL2/4 decreases the abundance of lignin in fibers and triggers an hypermethylation of glucuronoxylan that is linked to an over-accumulation of GlucuronoXylan Methyltransferase1/3 (GXM1/3) proteins. We demonstrate that MSIL4 binds to the GXM1/3 mRNAs, likely repressing their translation in wild-type plants. Our results reveal cell-type-specific mechanisms underlying SCW formation in Arabidopsis and point to a novel aspect of SCW regulation linking translational repression to regulation of glucuronoxylan methylation.
HostingRepositoryPRIDE
AnnounceDate2023-09-28
AnnouncementXMLSubmission_2023-09-28_09:10:25.457.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD040207
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterLaurianeKuhn
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListphosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-02-16 14:54:02ID requested
12023-09-28 09:10:25announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Arabidopsis, Musashi-type transcriptional regulator,cell wall, interactomic
Contact List
DominiqueGagliardi
contact affiliationInstitut de biologie moléculaire des plantes, CNRS, Université de Strasbourg, Strasbourg, France
contact emaildominique.gagliardi@ibmp-cnrs.unistra.fr
lab head
LaurianeKuhn
contact affiliationCNRS, IBMC, FRC1589
contact emaill.kuhn@ibmc-cnrs.unistra.fr
dataset submitter
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Dataset FTP location
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