PXD040207

PXD040207 is an original dataset announced via ProteomeXchange.

Title	Cell type-specific control of secondary cell wall formation by Musashi-type translational regulators in Arabidopsis
Description	Deciphering the mechanism of secondary cell wall/SCW formation in vascular plants is key to understanding their development and the molecular basis of biomass recalcitrance. A sophisticated network of transcription factors has been implicated in SCW synthesis in plants, but little is known about the implication of RNA-binding proteins in this process. Here we report that two RNA-binding proteins homologous to the animal translational regulator Musashi, Musashi-Like2/MSIL2 and Musashi-Like4/MSIL4, function redundantly to control SCW formation in interfascicular fibers and the setting of biomass recalcitrance. We show that the disruption of MSIL2/4 decreases the abundance of lignin in fibers and triggers an hypermethylation of glucuronoxylan that is linked to an over-accumulation of GlucuronoXylan Methyltransferase1/3 (GXM1/3) proteins. We demonstrate that MSIL4 binds to the GXM1/3 mRNAs, likely repressing their translation in wild-type plants. Our results reveal cell-type-specific mechanisms underlying SCW formation in Arabidopsis and point to a novel aspect of SCW regulation linking translational repression to regulation of glucuronoxylan methylation.
HostingRepository	PRIDE
AnnounceDate	2023-09-28
AnnouncementXML	Submission_2023-09-28_09:10:25.457.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD040207
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	LaurianeKuhn
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-16 14:54:02	ID requested
⏵ 1	2023-09-28 09:10:25	announced

Dataset with its publication pending

submitter keyword: Arabidopsis, Musashi-type transcriptional regulator,cell wall, interactomic

DominiqueGagliardi
contact affiliation	Institut de biologie moléculaire des plantes, CNRS, Université de Strasbourg, Strasbourg, France
contact email	dominique.gagliardi@ibmp-cnrs.unistra.fr
lab head
LaurianeKuhn
contact affiliation	CNRS, IBMC, FRC1589
contact email	l.kuhn@ibmc-cnrs.unistra.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/09/PXD040207

PRIDE project URI

• PRIDE
  1. PXD040207
     1. Label: PRIDE project
     2. Name: Cell type-specific control of secondary cell wall formation by Musashi-type translational regulators in Arabidopsis