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PXD040191-2

PXD040191 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA Lysosome membrane regeneration pathway depends on TBC1D15 and autophagic lysosomal reformation proteins
DescriptionAcute lysosomal membrane damage reduces the cellular population of functional lysosomes. However, these damaged lysosomes have a remarkable recovery potential independent of lysosomal biogenesis and remain unaffected in TFEB/TFE3-depleted cells. We combined proximity labelling based proteomics, biochemistry and high-resolution microscopy to unravel a new lysosomal membrane regeneration pathway which is dependent on ATG8, lysosomal membrane protein LIMP2, the Rab7 GAP TBC1D15, and proteins required for autophagic lysosomal reformation (ALR) including Dynamin2, Kinesin5B and Clathrin. Upon lysosomal damage, LIMP2 act as a lysophagy receptor to bind ATG8, which in turn recruits TBC1D15 to damaged membranes. TBC1D15 hydrolyses Rab7-GTP to segregate the damaged lysosomal mass and provides a scaffold to assemble and stabilize the ALR machinery, potentiating the formation of lysosomal tubulesand subsequent Dynamin2-dependent scission. TBC1D15-mediated lysosome regeneration was also observed in a cell culture model of oxalate nephropathy.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_09:00:36.777.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAnshu Bhattacharya
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF; Orbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-02-16 14:02:55ID requested
12023-03-07 05:30:56announced
22023-11-14 09:00:37announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: autophagy, lysosomal regeneration,lysosomes, autophagic lysosomal reformation, TBC family of proteins
Contact List
Ivan Dikic
contact affiliationInstitute of Biochemistry II; University Hospital of Goethe University Frankfurt Building 75 ,Ground floor, Room 0.205 Theodor-Stern-Kai 7 60590 Frankfurt am Main, Germany
contact emaildikic@biochem2.uni-frankfurt.de
lab head
Anshu Bhattacharya
contact affiliationGoethe University, Institute of Biochemistry 2, Buchmann Institute For Molecular Life Sciences
contact emailbhattacharya@med.uni-frankfurt.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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