PXD040074-1
PXD040074 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proximity labeling of OsPUX8-2.2 interacting proteins with TurboID |
| Description | Ubiquitin regulatory X (UBX) domain-containing proteins are one of the major subgroups of cofactors of the AAA+ ATPase CDC48/p97 and function in protein quality control involved in diverse cellular processes. However, little is known whether and how the UBX-containing proteins participate in host-microbe interaction. Here, we report a host nucleus-localized effector from Magnaporthe oryzae, MoNLE1, functions as a virulence factor and suppresses rice immunity by specially interferes with a rice UBX-containing (PUX) protein OsPUX8-2.2. To reveal the working mechanism of OsPUX8-2.2, we performed TurboID-based proximity labeling experiments to probe OsPUX8-2.2 interaction proteins. The result showed that OsPUX8-2.2 associates with a set of proteins involved in RNA processing and modification, protein folding and heat/light responses. We verified that a SGS domain-containing protein OsBHT, which negatively regulates rice defense against blast pathogen, is targeted by OsPUX8-2.2-OsCDC48-6 for subsequent degradation. |
| HostingRepository | iProX |
| AnnounceDate | 2023-02-12 |
| AnnouncementXML | Submission_2023-02-12_19:26:44.900.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Xuetao Shi |
| SpeciesList | scientific name: Oryza sativa; NCBI TaxID: 4530; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive; timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-02-12 19:26:29 | ID requested | |
| ⏵ 1 | 2023-02-12 19:26:45 | announced | |
| 2 | 2024-03-27 20:43:34 | announced | 2024-03-28: Update information. |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Rice (Oryza sativa japonica), Magnaporthe oryzae, effector, UBX domain, MoNLE1, OsPUX8-2.2, OsCDC48-6, OsBHT |
Contact List
| Wende Liu | |
|---|---|
| contact affiliation | Institute of Plant Protection, Chinese Academy of Agricultural Sciences |
| contact email | liuwende@caas.cn |
| lab head | |
| Xuetao Shi | |
| contact affiliation | Institute of Plant Protection, Chinese Academy of Agricultural Sciences |
| contact email | shixuetao@caas.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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