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PXD040074

PXD040074 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProximity labeling of OsPUX8B.2 interacting proteins with TurboID
DescriptionUbiquitin regulatory X (UBX) domain-containing proteins are one of the major subgroups of cofactors of the AAA+ ATPase CDC48/p97 and function in protein quality control involved in diverse cellular processes. However, little is known whether and how the UBX-containing proteins participate in host-microbe interaction. Here, we report a host nucleus-localized effector from Magnaporthe oryzae, MoNLE1, functions as a virulence factor and suppresses rice immunity by specially interferes with a rice UBX-containing (PUX) protein OsPUX8B.2. To reveal the working mechanism of OsPUX8B.2, we performed TurboID-based proximity labeling experiments to probe OsPUX8B.2 interaction proteins. The result showed that OsPUX8B.2 associates with a set of proteins involved in RNA processing and modification, protein folding and heat/light responses. We verified that a SGS domain-containing protein OsBHT, which negatively regulates rice defense against blast pathogen, is targeted by OsPUX8B.2-OsCDC48-6 for subsequent degradation.
HostingRepositoryiProX
AnnounceDate2023-05-23
AnnouncementXMLSubmission_2024-03-27_20:43:33.448.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterXuetao Shi
SpeciesList scientific name: Oryza sativa; NCBI TaxID: 4530;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive; timsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-02-12 19:26:29ID requested
12023-02-12 19:26:45announced
22024-03-27 20:43:34announced2024-03-28: Update information.
Publication List
Shi X, Xie X, Guo Y, Zhang J, Gong Z, Zhang K, Mei J, Xia X, Xia H, Ning N, Xiao Y, Yang Q, Wang GL, Liu W, A fungal core effector exploits the OsPUX8B.2-OsCDC48-6 module to suppress plant immunity. Nat Commun, 15(1):2559(2024) [pubmed]
Keyword List
submitter keyword: Rice (Oryza sativa japonica), Magnaporthe oryzae, effector, UBX domain, MoNLE1, OsPUX8B.2, OsCDC48-6, OsBHT
Contact List
Wende Liu
contact affiliationInstitute of Plant Protection, Chinese Academy of Agricultural Sciences
contact emailliuwende@caas.cn
lab head
Xuetao Shi
contact affiliationInstitute of Plant Protection, Chinese Academy of Agricultural Sciences
contact emailshixuetao@caas.cn
dataset submitter
Full Dataset Link List
iProX dataset URI