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PXD039855-1

PXD039855 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleReal-time spectral library matching for sample multiplexed quantitative proteomics
DescriptionSample multiplexed quantitative proteomics has proved to be a highly versatile means to assay molecular phenotypes. Yet, stochastic precursor selection and precursor coisolation can dramatically reduce the efficiency of data acquisition and quantitative accuracy. To address this, intelligent data acquisition (IDA) strategies have recently been developed to improve instrument efficiency and quantitative accuracy for both discovery and targeted methods. To this end we sought to develop and implement a new real-time library searching (RTLS) workflow that could enable intelligent scan triggering and peak selection within milliseconds of scan acquisition. To ensure ease of use and general applicability, we built an application to read in diverse spectral libraries and file types from both empirical and predicted spectral libraries. We demonstrate that RTLS methods enable improved quantitation of multiplexed samples, particularly with consideration for quantitation from chimeric fragment spectra. We used RTLS to profile proteome responses to small molecule perturbations and were able to quantify up to 15% more significantly regulated proteins in half the gradient time as traditional methods.
HostingRepositoryMassIVE
AnnounceDate2024-01-31
AnnouncementXMLSubmission_2024-01-31_12:30:31.498.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterChris McGann
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; scientific name: Saccharomyces cerevisiae; common name: baker's yeast; NCBI TaxID: 4932;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Eclipse; instrument model
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-02-03 17:15:05ID requested
12024-01-31 12:30:31announced
Publication List
no publication
Keyword List
submitter keyword: Proteomics, Real-time library search, Intelligent data acquisition
Contact List
Devin Schweppe
contact affiliationUniversity of Washington
contact emaildkschwep@uw.edu
lab head
Chris McGann
contact affiliationUniversity of Washington
contact emailcmcgann@uw.edu
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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