PXD039855
PXD039855 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Real-time spectral library matching for sample multiplexed quantitative proteomics |
| Description | Sample multiplexed quantitative proteomics has proved to be a highly versatile means to assay molecular phenotypes. Yet, stochastic precursor selection and precursor coisolation can dramatically reduce the efficiency of data acquisition and quantitative accuracy. To address this, intelligent data acquisition (IDA) strategies have recently been developed to improve instrument efficiency and quantitative accuracy for both discovery and targeted methods. To this end we sought to develop and implement a new real-time library searching (RTLS) workflow that could enable intelligent scan triggering and peak selection within milliseconds of scan acquisition. To ensure ease of use and general applicability, we built an application to read in diverse spectral libraries and file types from both empirical and predicted spectral libraries. We demonstrate that RTLS methods enable improved quantitation of multiplexed samples, particularly with consideration for quantitation from chimeric fragment spectra. We used RTLS to profile proteome responses to small molecule perturbations and were able to quantify up to 15% more significantly regulated proteins in half the gradient time as traditional methods. |
| HostingRepository | MassIVE |
| AnnounceDate | 2024-01-31 |
| AnnouncementXML | Submission_2024-01-31_12:30:31.498.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Chris McGann |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; scientific name: Saccharomyces cerevisiae; common name: baker's yeast; NCBI TaxID: 4932; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Eclipse; instrument model |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-02-03 17:15:05 | ID requested | |
| ⏵ 1 | 2024-01-31 12:30:31 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Proteomics, Real-time library search, Intelligent data acquisition |
Contact List
| Devin Schweppe | |
|---|---|
| contact affiliation | University of Washington |
| contact email | dkschwep@uw.edu |
| lab head | |
| Chris McGann | |
| contact affiliation | University of Washington |
| contact email | cmcgann@uw.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000091200/ |
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