<<< Full experiment listing

PXD039854-1

PXD039854 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of TTC29 protein partners in mouse testis
DescriptionWe previously identified gene variants in TTC29 that induce human male infertility due to asthenozoospermia and MMAF phenotype (Lores et al. Am J hum genet 2019). By means of CRISPR/Cas9 mutagenesis we demonstrated that the orthologous TTC29 protein in mouse also constitute a flagellar protein that is required for proper beating of the flagella, indicating an evolutionary-conserved function of TTC29. In here, we took advantage of the mutant mouse model to further decipher the protein complexes associated with TTC29 function within the flagella. We performed co-immunoprecipitation experiments on testis protein extracts from wild-type mice using TTC29 specific antibodies and performed comparative mass spectrometry analyses with two TTC29 KO mouse lines we previously generated and shown to lack TTC29 protein (Ttc29-/- L5 and L7) (Lores et al. Am J hum genet 2019. Mass spectrometry analyses were performed on four independent experimental replicates for each mouse line.
HostingRepositoryPRIDE
AnnounceDate2023-11-06
AnnouncementXMLSubmission_2023-11-06_07:28:47.217.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJohannaBRUCE
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListacetylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-02-03 15:37:22ID requested
12023-11-06 07:28:47announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: co-immunoprecipitation, asthenozoospermia, male infertility,TTC29, Crispr mouse model
Contact List
PatrickLORES
contact affiliationUniversité de Paris, Institut Cochin, U1016, INSERM, CNRS, Paris, France.
contact emailpatrick.lores@inserm.fr
lab head
JohannaBRUCE
contact affiliationProteom’IC facility, Université Paris Cité, CNRS, INSERM, Institut Cochin, F-75014 PARIS
contact emailjohanna.bruce@inserm.fr
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/11/PXD039854
PRIDE project URI
Repository Record List
[ + ]