PXD039780 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of proteins crosslinked to lysosomal sphingosine and cholesterol probes |
Description | HeLa cells were labelled with lysosomally localized photocrosslinkable and clickable probes for sphingosine and cholesterol. Upon photocrosslinking and cell lysis, the protein-lipid complexes were clicked to biotin azide and enriched via Streptavidin-immunoprecipitation. Enriched protein-lipid complexes were then analyzed by LC-MS/MS to identify proteins interacting with sphingosine and cholesterol, respectively. |
HostingRepository | PRIDE |
AnnounceDate | 2023-06-29 |
AnnouncementXML | Submission_2023-06-29_06:33:05.709.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | FrankStein |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-02-01 05:21:29 | ID requested | |
1 | 2023-02-07 02:11:17 | announced | |
⏵ 2 | 2023-06-29 06:33:06 | announced | 2023-06-29: Updated project metadata. |
3 | 2023-11-14 09:01:45 | announced | 2023-11-14: Updated project metadata. |
Publication List
Altuzar J, Notbohm J, Stein F, Haberkant P, Hempelmann P, Heybrock S, Worsch J, Saftig P, H, รถ, glinger D, Lysosome-targeted multifunctional lipid probes reveal the sterol transporter NPC1 as a sphingosine interactor. Proc Natl Acad Sci U S A, 120(11):e2213886120(2023) [pubmed] |
Keyword List
submitter keyword: lysosomal lipids,photoaffinity labelling, click chemistry |
Contact List
Dr. DorisHoeglinger |
contact affiliation | Heidelberg University Biohemistry Center |
contact email | doris.hoeglinger@bzh.uni-heidelberg.de |
lab head | |
FrankStein |
contact affiliation | EMBL |
contact email | frank.stein@embl.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/02/PXD039780 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD039780
- Label: PRIDE project
- Name: Identification of proteins crosslinked to lysosomal sphingosine and cholesterol probes