PXD039780

PXD039780 is an original dataset announced via ProteomeXchange.

Title	Identification of proteins crosslinked to lysosomal sphingosine and cholesterol probes
Description	HeLa cells were labelled with lysosomally localized photocrosslinkable and clickable probes for sphingosine and cholesterol. Upon photocrosslinking and cell lysis, the protein-lipid complexes were clicked to biotin azide and enriched via Streptavidin-immunoprecipitation. Enriched protein-lipid complexes were then analyzed by LC-MS/MS to identify proteins interacting with sphingosine and cholesterol, respectively.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:01:43.694.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Frank Stein
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-01 05:21:29	ID requested
1	2023-02-07 02:11:17	announced
2	2023-06-29 06:33:06	announced	2023-06-29: Updated project metadata.
⏵ 3	2023-11-14 09:01:45	announced	2023-11-14: Updated project metadata.

Altuzar J, Notbohm J, Stein F, Haberkant P, Hempelmann P, Heybrock S, Worsch J, Saftig P, H, ö, glinger D, Lysosome-targeted multifunctional lipid probes reveal the sterol transporter NPC1 as a sphingosine interactor. Proc Natl Acad Sci U S A, 120(11):e2213886120(2023) [pubmed]

submitter keyword: lysosomal lipids,photoaffinity labelling, click chemistry

Dr. Doris Hoeglinger
contact affiliation	Heidelberg University Biohemistry Center
contact email	doris.hoeglinger@bzh.uni-heidelberg.de
lab head
Frank Stein
contact affiliation	EMBL
contact email	frank.stein@embl.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/02/PXD039780

PRIDE project URI

• PRIDE
  1. PXD039780
     1. Label: PRIDE project
     2. Name: Identification of proteins crosslinked to lysosomal sphingosine and cholesterol probes