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PXD039664-1

PXD039664 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTetraspanin 4 stabilizes membrane swellings and facilitates their maturation into migrasomes
DescriptionMigrasomes are newly discovered cell organelles forming by local swelling of retraction fibers. The migrasome formation critically depends on tetraspanin proteins present in the retraction fiber membranes and is modulated by the membrane tension and bending rigidity. It remained unknown how and in which time sequence these factors are involved in migrasome nucleation, growth, and stabilization, and what are the possible intermediate stages of migrasome biogenesis. Here using live cell imaging and biomimetic system for migrasomes and retraction fibers, we reveal that migrasome formation is a two-stage process. At the first stage, which in biomimetic system is mediated by membrane tension, local swellings largely devoid of tetraspanin 4 form on the retraction fibers. At the second stage, tetraspanin 4 molecules migrate toward and onto these swellings, which grow up to several microns in size and transform into migrasomes. This tetraspanin 4 recruitment to the swellings is essential for migrasome growth and stabilization. Based on these findings we propose that the major role of tetraspanin proteins is in stabilizing the migrasome structure while the migrasome nucleation and initial growth stages can be driven by membrane mechanical stresses.
HostingRepositoryPRIDE
AnnounceDate2023-01-29
AnnouncementXMLSubmission_2023-01-28_23:56:01.707.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTamarZiv
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-01-25 08:16:57ID requested
12023-01-28 23:56:02announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Migrasome,Tetraspanin 4
Contact List
RayaSorkin
contact affiliationSchool of Chemistry, Raymond & Beverly Sackler Faculty of Exact Sciences and Center for Physics and Chemistry of Living Systems, Tel Aviv University, Tel Aviv, Israel
contact emailrsorkin@tauex.tau.ac.il
lab head
TamarZiv
contact affiliationTechnion
contact emailtamarz@technion.ac.il
dataset submitter
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Dataset FTP location
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