PXD039403-1

PXD039403 is an original dataset announced via ProteomeXchange.

Title	RNA Helicase DDX3X Modulates Herpes Simplex Virus 1 Nuclear Egress
Description	DDX3X is a mammalian RNA helicase that regulates RNA metabolism, cancers, innate immunity and several RNA viruses. We discovered that herpes simplex virus 1, a nuclear DNA replicating virus, highjacks redirects DDX3X to the nuclear envelope where it surprisingly modulates the exit of newly assembled viral particles. DDX3X depletion also led to an accumulation of virions in intranuclear herniations. Mechanistically, we show that DDX3X physically and functionally interacts with the virally encoded nuclear egress complex at the inner nuclear membrane. DDX3X also bound to and stimulated the incorporation in mature particles of pUs3, a herpes kinase that promotes viral nuclear release across the outer nuclear membrane. Overall, the data highlights two unexpected roles for an RNA helicase during the passage of herpes simplex viral particles through the nuclear envelope. This reveals a highly complex interaction between DDX3X and viruses and provides new opportunities to target viral propagation.
HostingRepository	PRIDE
AnnounceDate	2023-02-21
AnnouncementXML	Submission_2023-02-21_12:09:48.888.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD039403
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	EricBonneil
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-13 06:05:00	ID requested
⏵ 1	2023-02-21 12:09:49	announced
2	2023-11-14 07:37:40	announced	2023-11-14: Updated project metadata.

10.6019/PXD039403;

Khadivjam B, Bonneil É, Thibault P, Lipp, é R, RNA helicase DDX3X modulates herpes simplex virus 1 nuclear egress. Commun Biol, 6(1):134(2023) [pubmed]

submitter keyword: herpesvirus, NEC, HSV, helicase,herpes, DDX, DDX3, host-pathogen interaction, US3, UL31

RogerLippe
contact affiliation	Centre de Recherche du CHU Ste-Justine
contact email	roger.lippe@umontreal.ca
lab head
EricBonneil
contact affiliation	Proteomic Platform
contact email	eric.bonneil@umontreal.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/02/PXD039403

PRIDE project URI

• PRIDE
  1. PXD039403
     1. Label: PRIDE project
     2. Name: RNA Helicase DDX3X Modulates Herpes Simplex Virus 1 Nuclear Egress