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PXD039363-1

PXD039363 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIlluminating phenotypic drug responses of sarcoma cells to kinase inhibitors by phosphoproteomics
DescriptionKinase inhibitors (KIs) are important cancer drugs but often display polypharmacology that is molecularly not understood. This disconnect is particularly apparent in cancer entities such as sarcomas for which the oncogenic drivers are often not clear. To investigate more systematically how the cellular proteotypes of sarcoma cells shape their response to molecularly targeted drugs, we profiled the proteomes and phosphoproteomes of 17 sarcoma cell lines and screened the same cells against 150 cancer drugs. The resulting 2,550 phenotypic drug profiles revealed distinct drug responses and the cellular activity landscapes derived from deep (phospho)proteomes (9-10,000 proteins and 10-27,000 phosphorylation sites per cell line) enabled several lines of analysis. For instance, connecting the (phospho)proteomic data with drug responses revealed known and novel mechanisms of action (MoAs) of kinase inhibitors and identified markers of drug sensitivity or resistance. All data is publically accessible via an interactive web application that enables exploration of this rich molecular resource for better understanding active signalling pathways in sarcoma cells, identifying treatment response predictors, and revealing novel MoA of clinical KIs.
HostingRepositoryMassIVE
AnnounceDate2023-01-12
AnnouncementXMLSubmission_2023-01-12_01:52:15.971.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterChien-Yun Lee
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationListTMT6plex; phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-01-11 07:52:15ID requested
12023-01-12 01:52:16announced
Publication List
no publication
Keyword List
submitter keyword: proteotypes, phosphoproteomics, kinase inhibitor, sarcoma, drug response
Contact List
Bernhard Kuster
contact affiliationChair of Proteomics and Bioanalytics, School of Life Sciences, Technical University of Munich
contact emailkuster@tum.de
lab head
Chien-Yun Lee
contact affiliationTechnical University of Munich
contact emailchienyun.lee@tum.de
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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