PXD039318 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Oxidized methionine melanoma epitopes |
Description | One of the most promising alternatives to chemotherapy in cancer treatment is immunotherapy, particularly, peptide-based immunotherapy in which synthetic peptides derived from tumor-specific antigens are used to trigger an immune response to specifically target cancer cells. Here using custom nanoLC-MS/MS based workflows we examine the possible presentation of peptide-based oxidative variants in complex with Major Histocompatibility Complex (MHC) class I proteins at the surface of melanoma cells. The deposited data contains the raw files associated with mild acidic elutions (MAE) of MHC class I associated peptides from various melanoma cell lines. These were analysed using Data Independent Acquisition (DIA), employing a custom method for MS/MS fragmentation of the native and Met-oxidative modifications of a tyrosinase derived peptide (YMDGTMSQV denoted as YMD peptide). The data contains also the raw files referring to the nanoLC-MS/MS analysis of HPLC purified fractions of various oxidative derivatives of the YMD peptide but also of MAGE-10 native and oxidized forms. |
HostingRepository | PRIDE |
AnnounceDate | 2023-07-25 |
AnnouncementXML | Submission_2023-07-25_05:11:30.129.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | CristianMunteanu |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | dihydroxylated residue; monohydroxylated residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-01-09 02:07:19 | ID requested | |
⏵ 1 | 2023-07-25 05:11:31 | announced | |
2 | 2023-11-14 09:04:26 | announced | 2023-11-14: Updated project metadata. |
Publication List
Chiri, ţ, oiu GN, Munteanu CVA, Ş, ulea TA, Spiridon L, Petrescu AJ, Jandus C, Romero P, Petrescu Ş M, Methionine oxidation selectively enhances T cell reactivity against a melanoma antigen. iScience, 26(7):107205(2023) [pubmed] |
Keyword List
submitter keyword: peptidomics, mass spectrometry, tyrosinase, immunotherapy,melanoma |
Contact List
Ștefana M.Petrescu |
contact affiliation | Dept. of Molecular Cell Biology of the Institute of Biochemistry of the Romanian Academy |
contact email | stefana.petrescu@biochim.ro |
lab head | |
CristianMunteanu |
contact affiliation | Institute of Biochemistry of the Romanian Academy (IBRA) |
contact email | cristian.v.a.munteanu@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD039318 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD039318
- Label: PRIDE project
- Name: Oxidized methionine melanoma epitopes