PXD039318

PXD039318 is an original dataset announced via ProteomeXchange.

Title	Oxidized methionine melanoma epitopes
Description	One of the most promising alternatives to chemotherapy in cancer treatment is immunotherapy, particularly, peptide-based immunotherapy in which synthetic peptides derived from tumor-specific antigens are used to trigger an immune response to specifically target cancer cells. Here using custom nanoLC-MS/MS based workflows we examine the possible presentation of peptide-based oxidative variants in complex with Major Histocompatibility Complex (MHC) class I proteins at the surface of melanoma cells. The deposited data contains the raw files associated with mild acidic elutions (MAE) of MHC class I associated peptides from various melanoma cell lines. These were analysed using Data Independent Acquisition (DIA), employing a custom method for MS/MS fragmentation of the native and Met-oxidative modifications of a tyrosinase derived peptide (YMDGTMSQV denoted as YMD peptide). The data contains also the raw files referring to the nanoLC-MS/MS analysis of HPLC purified fractions of various oxidative derivatives of the YMD peptide but also of MAGE-10 native and oxidized forms.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:04:18.572.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cristian Munteanu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	dihydroxylated residue; monohydroxylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-09 02:07:19	ID requested
1	2023-07-25 05:11:31	announced
⏵ 2	2023-11-14 09:04:26	announced	2023-11-14: Updated project metadata.

Chiri, ţ, oiu GN, Munteanu CVA, Ş, ulea TA, Spiridon L, Petrescu AJ, Jandus C, Romero P, Petrescu Ş M, Methionine oxidation selectively enhances T cell reactivity against a melanoma antigen. iScience, 26(7):107205(2023) [pubmed]

submitter keyword: peptidomics, mass spectrometry, tyrosinase, immunotherapy,melanoma

Ștefana M. Petrescu
contact affiliation	Dept. of Molecular Cell Biology of the Institute of Biochemistry of the Romanian Academy
contact email	stefana.petrescu@biochim.ro
lab head
Cristian Munteanu
contact affiliation	Institute of Biochemistry of the Romanian Academy (IBRA)
contact email	cristian.v.a.munteanu@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD039318

PRIDE project URI

• PRIDE
  1. PXD039318
     1. Label: PRIDE project
     2. Name: Oxidized methionine melanoma epitopes