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PXD039270-2

PXD039270 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleUSING DESIGN OF EXPERIMENTS (DoE) TO OPTIMIZE PERFORMANCE AND STABILITY OF BIOMIMETIC CELL MEMBRANE-COATED NANOSTRUCTURES FOR CANCER THERAPY
DescriptionThe present study aimed to develop and optimize cell derived membrane-coated nanostructures by applying a design of experiments (DoE) to improve the therapeutic index by homotypic targeting in cancer cells. The protein composition of the extracted cell membrane from tumoral cells were assessed by mass spectrometry-based proteomics. PLGA-based nanoparticles encapsulating temozolomide (TMZ NPs) were successfully developed. The coating technology applying the isolated U251 cell membrane (MB) was optimized using a fractional two-level three-factor factorial design. All the formulation runs were systematically characterized regarding their diameter, polydispersity index (PDI), and zeta potential (ZP). Experimental conditions generated by DoE were also subjected to morphological studies using negative-staining transmission electron microscopy (TEM). MicroRaman, Fourier-Transform Infrared (FTIR) spectroscopies and Confocal microscopy were used as characterization techniques for evaluating the NP-MB nanostructures. Internalization studies were carried out to evaluate the homotypic targeting ability. The results have shown that nearly 80% of plasma membrane proteins were retained in the cell membrane vesicles after the isolation process, including key proteins to the homotypic binding. DoE analysis considering acquired TEM images reveals that condition run five should be the best-optimized procedure to produce the biomimetic cell-derived membrane-coated nanostructure (NP-MB). Raman, FTIR, and confocal characterization results have shown the successful encapsulation of TMZ drug and provided evidence of the effective coating applying the MB. Cell internalization studies corroborate the proteomic data indicating that the optimized NP-MB achieved specific targeting of homotypic tumor cells. The structure should retain the complex biological functions of U251 natural cell membranes while exhibiting physicochemical properties suitable for effective homotypic recognition. Together, these findings provide coverage and a deeper understanding regarding the dynamics around extracted cell membrane and polymeric nanostructures interactions and an in-depth insight into the cell membrane coating technology and the development of optimized biomimetic and bioinspired nanostructured systems.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_09:00:07.052.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterNatália Ferreira
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumenttimsTOF Pro; LTQ
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-01-05 02:13:38ID requested
12023-01-30 17:12:59announced
22023-11-14 09:00:07announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: cell membrane coating technology
mass spectrometry-based proteomics
biomimetic systems
stability
design of experiments
homotypic recognition
glioblastoma
temozolomide drug.
Contact List
Valtencir Zucolotto
contact affiliationNanomedicine and Nanotoxicology Group, Physics Institute of São Carlos, São Paulo University. Avenida Trabalhador São Carlense, 400, CEP13560970 (Brazil) Phone: (+55) 16 3373 8656, São Carlos – SP – Brazil.
contact emailzuco@ifsc.usp.br
lab head
Natália Ferreira
contact affiliationNanomedicine and Nanotoxicology Group, Physics Institute of São Carlos, São Paulo University. Avenida Trabalhador São Carlense, 400, CEP13560970 (Brazil) Phone: (+55) 16 3373 8656, São Carlos – SP – Brazil.
contact emailnatalia.noronha@usp.br
dataset submitter
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