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PXD039123-1

PXD039123 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleVerticillium dahliae Vta3 promotes ELV1 virulence factor gene expression in xylem sap, but tames Mtf1-mediated late stages of fungus-plant interactions and microsclerotia formation
DescriptionVerticillium transcription activator of adhesion 3 (Vta3) is required for plant root colonization and pathogenicity of the soil-borne vascular fungus Verticillium dahliae [Bui et al., 2018]. In this study, we discovered that Vta3 contributes to the virulence of V. dahliae on tomato plants by promoting ELV1 gene expression, which is not necessary for vegetative growth or production of conidiospores. Gene expression of the transcription factor Mtf1 is reduced in the presence of an intact VTA3 gene. The presence of an intact MTF1 gene triggers the expression of fungal effector genes and plant immune responses (transcription of tomato pathogenesis-related protein genes, and levels of pipecolic and salicylic acids functioning in tomato defense signaling against (hemi-) biotrophic pathogens), and is required for disease symptoms similar to those seen in tomato plants infected by the V. dahliae wild-type. Mtf1 promotes the formation of resting structures at the end of the infection cycle, which allows the fungus to survive in the soil and later to re-infect host plants. Protein pull-downs were performed with GFP-fused Vta3 to investigate whether there is evidence for a direct interaction of the regulators Vta3 and Mtf1. In this study, ribosomal proteins predominantly co-enriched with Vta3, and our data set did not give evidence that Mtf1 directly interacts with Vta3. Bui T-T, Harting R, Braus-Stromeyer SA, Tran V-T, Leonard M, Höfer A, et al. Verticillium dahliae transcription factors Som1 and Vta3 control microsclerotia formation and sequential steps of plant root penetration and colonisation to induce disease. New Phytologist. 2019;221: 2138–2159. doi:10.1111/nph.15514
HostingRepositoryPRIDE
AnnounceDate2023-01-18
AnnouncementXMLSubmission_2023-01-18_03:07:18.624.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterOliverValerius
SpeciesList scientific name: Verticillium dahliae JR2; NCBI TaxID: 1202531;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-01-02 01:11:58ID requested
12023-01-18 03:07:19announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Verticillium dahliae, Master transcription factor 1, JR2, Mtf1, transcription factor, Vta3, Verticillium transcription activator of adhesion 3
Contact List
Gerhard H.Braus
contact affiliationDepartment of Molecular Microbiology and Genetics, Institute of Microbiology and Genetics and Goettingen Center for Molecular Biosciences (GZMB), University of Goettingen, Germany
contact emailgbraus@gwdg.de
lab head
OliverValerius
contact affiliationGeorg-August-Universität Göttingen
contact emailovaleri@gwdg.de
dataset submitter
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