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PXD038964-1

PXD038964 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleComparative phyloproteomics identifies conserved plasmodesmal proteins
DescriptionPlasmodesmata are cytosolic bridges, lined by the plasma membrane and traversed by endoplasmic reticulum, connecting cells and tissues, and critical for many aspects of plant biology. While plasmodesmata are notoriously difficult to extract, tissue fractionation and proteomic analyses can yield valuable knowledge of their composition. Here we have generated two novel proteomes to expand tissue and taxonomic representation of plasmodesmata: one from mature Arabidopsis leaves and one from the moss Physcomitrium patens and leveraged these and existing data to perform a comparative analysis to identify evolutionarily conserved protein families that are associated with plasmodesmata. Thus, we identified b-1,3-glucanases, C2 lipid-binding proteins and tetraspanins as core plasmodesmal components that likely serve as essential structural or functional components. Our approach has not only identified elements of a conserved plasmodesmal proteome, but also demonstrated the added power offered by comparative analysis for recalcitrant samples. Conserved plasmodesmal proteins establish a basis upon which ancient plasmodesmal function can be further investigated to determine the essential roles these structures play in multicellular organism physiology in the green lineages. 
HostingRepositoryPRIDE
AnnounceDate2023-01-13
AnnouncementXMLSubmission_2023-01-13_02:01:16.346.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMatthewJohnston
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; scientific name: Physcomitrella patens subsp. patens (Moss); NCBI TaxID: 3218;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-12-20 06:47:02ID requested
12023-01-13 02:01:16announced
22023-11-14 08:59:35announced2023-11-14: Updated project metadata.
Publication List
Keyword List
submitter keyword: Arabidopsis,plasmodesmata, phyloproteomics, Physcomitrium
Contact List
ChristineFaulkner
contact affiliationCrop Genetics John Innes Centre Norfolk United Kingdom
contact emailchristine.faulkner@jic.ac.uk
lab head
MatthewJohnston
contact affiliationCrop Genetics, John Innes Centre, Norwich, United Kingdom
contact emailmattjstn@outlook.com
dataset submitter
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Dataset FTP location
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