PXD038905 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The Triticale Mature Pollen and Stigma Proteomes – Assembling the Proteins for a Productive Encounter |
Description | Triticeae crops are major contributors to global food production and ensuring their capacity to reproduce and generate seeds is critical. However, despite their importance our knowledge of the proteins underlying Triticeae reproduction is severely lacking and this is not only true of pollen and stigma development, but also of their pivotal interaction. When the pollen grain and stigma are brought together they have each accumulated the proteins required for their intended meeting and accordingly studying their mature proteomes is bound to reveal proteins involved in their diverse and complex interactions. Using triticale as a Triticeae representative, gel-free shotgun proteomics was used to identify 11533 and 2977 mature stigma and pollen proteins respectively. These datasets, by far the largest to date, provide unprecedented insights into the proteins participating in Triticeae pollen and stigma development and interactions. The study of the Triticeae stigma has been particularly neglected and to begin filling this knowledge gap, a developmental iTRAQ analysis was performed revealing 647 proteins displaying differential abundance as the stigma matures in preparation for pollination. An in-depth comparison to an equivalent Brassicaceae analysis divulged both conservation and diversification in the makeup and function of proteins involved in the pollen and stigma encounter. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:17:08.268.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Renee Robinson |
SpeciesList | scientific name: x Triticosecale; NCBI TaxID: 49317; |
ModificationList | iTRAQ4plex-116 reporter+balance reagent acylated residue; dehydrated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-12-19 03:05:54 | ID requested | |
1 | 2023-03-08 15:45:49 | announced | |
⏵ 2 | 2023-11-14 08:17:09 | announced | 2023-11-14: Updated project metadata. |
Publication List
Robinson R, Sprott D, Couroux P, Routly E, Labb, é N, Xing T, Robert LS, The triticale mature pollen and stigma proteomes - assembling the proteins for a productive encounter. J Proteomics, 278():104867(2023) [pubmed] |
Keyword List
submitter keyword: proteomics, Brassica, mass spectrometry, pollination,triticale, Triticeae, stigma, pollen, iTRAQ |
Contact List
Dr. Laurian Robert |
contact affiliation | Agriculture and Agri-Food Canada, Ottawa Research and Development Centre. |
contact email | robertlaurian@hotmail.com |
lab head | |
Renee Robinson |
contact affiliation | Agriculture and Agri-Food Canada, Carleton University |
contact email | drreneerobinson@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD038905
- Label: PRIDE project
- Name: The Triticale Mature Pollen and Stigma Proteomes – Assembling the Proteins for a Productive Encounter