PXD038905

PXD038905 is an original dataset announced via ProteomeXchange.

Title	The Triticale Mature Pollen and Stigma Proteomes – Assembling the Proteins for a Productive Encounter
Description	Triticeae crops are major contributors to global food production and ensuring their capacity to reproduce and generate seeds is critical. However, despite their importance our knowledge of the proteins underlying Triticeae reproduction is severely lacking and this is not only true of pollen and stigma development, but also of their pivotal interaction. When the pollen grain and stigma are brought together they have each accumulated the proteins required for their intended meeting and accordingly studying their mature proteomes is bound to reveal proteins involved in their diverse and complex interactions. Using triticale as a Triticeae representative, gel-free shotgun proteomics was used to identify 11533 and 2977 mature stigma and pollen proteins respectively. These datasets, by far the largest to date, provide unprecedented insights into the proteins participating in Triticeae pollen and stigma development and interactions. The study of the Triticeae stigma has been particularly neglected and to begin filling this knowledge gap, a developmental iTRAQ analysis was performed revealing 647 proteins displaying differential abundance as the stigma matures in preparation for pollination. An in-depth comparison to an equivalent Brassicaceae analysis divulged both conservation and diversification in the makeup and function of proteins involved in the pollen and stigma encounter.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:17:08.268.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Renee Robinson
SpeciesList	scientific name: x Triticosecale; NCBI TaxID: 49317;
ModificationList	iTRAQ4plex-116 reporter+balance reagent acylated residue; dehydrated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-19 03:05:54	ID requested
1	2023-03-08 15:45:49	announced
⏵ 2	2023-11-14 08:17:09	announced	2023-11-14: Updated project metadata.

Robinson R, Sprott D, Couroux P, Routly E, Labb, é N, Xing T, Robert LS, The triticale mature pollen and stigma proteomes - assembling the proteins for a productive encounter. J Proteomics, 278():104867(2023) [pubmed]

submitter keyword: proteomics, Brassica, mass spectrometry, pollination,triticale, Triticeae, stigma, pollen, iTRAQ

Dr. Laurian Robert
contact affiliation	Agriculture and Agri-Food Canada, Ottawa Research and Development Centre.
contact email	robertlaurian@hotmail.com
lab head
Renee Robinson
contact affiliation	Agriculture and Agri-Food Canada, Carleton University
contact email	drreneerobinson@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD038905

PRIDE project URI

• PRIDE
  1. PXD038905
     1. Label: PRIDE project
     2. Name: The Triticale Mature Pollen and Stigma Proteomes – Assembling the Proteins for a Productive Encounter