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PXD038856-1

PXD038856 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleFunctional nanovesicles from human trophectodermal cells for embryo implantation
DescriptionExtracellular vesicles (EVs) are important mediators of embryo attachment and outgrowth critical for successful implantation. While EVs have garnered immense interest in their therapeutic potential in assisted reproductive technology by improving implantation success, their large-scale generation remains a major challenge. Here, we report a rapid and scalable production of nanovesicles (NVs) from human trophectoderm cells (hTSCs) via membrane extrusion; these NVs can be generated in approximately 6 hours with a 20-fold higher yield than EVs isolated from the same number of cells. NVs display similar biophysical traits (morphologically intact, spherical, 90-130 nm) to EVs, and are laden with hallmark players of implantation (ITGA2/V, ITGB1, PRDX1, SOD2, and MFGE8) that include cell-matrix adhesion and extracellular matrix organisation proteins. Functionally, NVs are readily taken up by low-receptive endometrial HEC1A cells and reprogram their proteome towards a receptive phenotype that support hTSC spheroid attachment. Moreover, a single dose treatment of NVs significantly enhanced adhesion and spreading of mouse embryo trophoblast on fibronectin matrix. We show functional potential of NVs in enhancing embryo implantation and highlight their rapid and scalable generation, amenable to clinical utility.
HostingRepositoryPRIDE
AnnounceDate2023-02-20
AnnouncementXMLSubmission_2023-02-19_16:40:35.491.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterdavidgreening
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-12-14 22:13:06ID requested
12023-02-19 16:40:35announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: proteomics, extrusion,Nanovesicles, extracellular vesicles, embryo implantation, endometrial receptivity
Contact List
DavidGreening
contact affiliationBaker Heart & Diabetes Inst.
contact emaildavid.greening@baker.edu.au
lab head
davidgreening
contact affiliationBaker Heart and Diabetes Inst.
contact emaildavid.greening@baker.edu.au
dataset submitter
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Dataset FTP location
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