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PXD038824-1

PXD038824 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA simple non-invasive method to collect soft tick saliva reveals differences in Ornithodoros moubata saliva composition between ticks infected and uninfected with Borrelia duttonii spirochetes
DescriptionWe developed a new simple method to assess the composition of proteins in the Ornithodoros moubata saliva. To collect naturally expectorated saliva from the ticks, we employed an artificial membrane feeding technique using a simple, chemically defined diet and submitted the collected native saliva samples for liquid chromatography-mass spectrometry (LC-MS) analysis. These experiments were conducted with groups of uninfected ticks as well as with B. duttonii infected ticks. The ticks exhibited a fair feeding response to the tested diet, engorgement rates reaching between 60-100% of ticks per feeding chamber. LC-MS analysis identified a total of 17 and 15 proteins in saliva samples from the uninfected and infected ticks respectively. Importantly, the analysis was sensitive enough to detect up to 9 different proteins in the saliva containing diet upon which as few as 6 nymphal ticks fed. Some of the proteins identified are well known for their immunomodulatory activity in a vertebrate host, whereas others were structural or “housekeeping” proteins and their finding in the naturally expectorated saliva confirms that they can be secreted and having some roles at the tick-host interface. Most notably, some of the proteins that have long been suspected as important for vector-pathogen interactions of Borrelia spirochetes were detected only in the samples from infected ticks, suggesting that their expression was altered by the persistent colonization of the tick’s salivary glands by spirochetes. The simple method described herein is an important addition to the toolbox available to study the vector-host-pathogen interactions in the rapidly feeding soft ticks.
HostingRepositoryPRIDE
AnnounceDate2023-01-10
AnnouncementXMLSubmission_2023-01-10_08:33:25.946.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFilipDyčka
SpeciesList scientific name: Borrelia; NCBI TaxID: NCBITaxon:138; scientific name: Ornithodoros moubata; NCBI TaxID: 6938;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumenttimsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-12-13 23:23:46ID requested
12023-01-10 08:33:26announced
22023-11-14 08:59:58announced2023-11-14: Updated project metadata.
Publication List
Keyword List
submitter keyword: Relapsing fever Borrelia, artificial membrane feeding, Ornithodoros moubata, Saliva, infected,tick, LC-MS Analysis, Borrelia duttonii, spirochetes
Contact List
Ryan O.Rego
contact affiliationInstitute of Parasitology, Centre for Biology, Academy of Science of the Czech Republic, Czech Republic Faculty of Science, University of South Bohemia in Ceske Budejovice, Czech Republic
contact emailryanrego@paru.cas.cz
lab head
FilipDyčka
contact affiliationUniversity of South Bohemia in Ceske Budejovice Faculty of Science Branisovska 1760 Ceske Budejovice
contact emaildycka@mail.muni.cz
dataset submitter
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Dataset FTP location
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