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PXD038751-1

PXD038751 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTrans-Golgi protein TVP23B regulates host-microbe interactions via Paneth cell homeostasis and Goblet cell glycosylation
DescriptionA key feature in intestinal immunity is the dynamic intestinal barrier, which separates the host from resident and pathogenic microbiota through a mucus gel impregnated with antimicrobial peptides. The mechanisms underlying the maintenance and function of this intestinal barrier are not completely understood. Using a mouse forward genetic screen for defects of intestinal homeostasis, we have found a mutation in Tvp23b, which conferred susceptibility to chemically induced and infectious colitis. Golgi apparatus membrane protein TVP23 homolog B (TVP23B) is a transmembrane protein conserved from yeast to humans. In the intestine, the protein is localized to the epithelium and its deficiency in the hematopoietic extrinsic compartment was essential to the colitis phenotype. We found that TVP23B controls the homeostasis of Paneth cells and function of goblet cells in vivo, leading to a decrease in antimicrobial peptides as well as a more penetrable mucus layer. As a result, Tvp23b-/- mice displayed decreased barrier function and a loss of host-microbe separation. TVP23B-deficient colonocytes have a loss of core-3 O-glycosylation of colonic proteins, which is the major O-glycosylation present on gel forming mucins. TVP23B binds with another Golgi protein, YIPF6, which is similarly critical for intestinal homeostasis. The Golgi proteomes of YIPF6 and TVP23B-deficient colonocytes have a common deficiency of several critical glycosylation enzymes, including those necessary for core-3 glycosylation of mucins. TVP23B is necessary for the formation of the sterile mucin layer of the intestine and its absence disturbs the balance of host and microbe in vivo.
HostingRepositoryMassIVE
AnnounceDate2023-07-20
AnnouncementXMLSubmission_2023-07-20_08:53:07.891.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJames Moresco
SpeciesList scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-12-12 09:01:51ID requested
12023-07-20 08:53:08announced
Publication List
no publication
Keyword List
submitter keyword: Dextran sodium sulfate (DSS), N-ethyl-N-nitrosourea (ENU), inflammatory bowel disease (IBD), glycosylation, Paneth Cell, goblet cell
Contact List
Emre E. Turer
contact affiliationUniversity of Texas Southwestern Medical Center
contact emailemre.turer@utsouthwestern.edu
lab head
James Moresco
contact affiliationUT Southwestern
contact emailjames.moresco@utsouthwestern.edu
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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