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PXD038699-1

PXD038699 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSpatial single-cell mass spectrometry defines zonation of the hepatocyte proteome
DescriptionSingle-cell proteomics by mass spectrometry (MS) is emerging as a powerful and unbiased method for the characterization of biological heterogeneity. So far, it has been limited to cultured cells, whereas an expansion of the method to complex tissues would greatly enhance biological insights. Here we describe single-cell Deep Visual Proteomics (scDVP), a technology that integrates high-content imaging, laser microdissection and multiplexed MS. scDVP resolves the context-dependent, spatial proteome of murine hepatocytes at a current depth of 1,700 proteins from a slice of a cell. Half of the proteome was differentially regulated in a spatial manner, with protein levels changing dramatically in proximity to the central vein. We applied machine learning to proteome classes and images, which subsequently inferred the spatial proteome from imaging data alone. scDVP is applicable to healthy and diseased tissues and complements other spatial proteomics or spatial omics technologies.
HostingRepositoryPRIDE
AnnounceDate2023-10-03
AnnouncementXMLSubmission_2023-10-03_10:35:16.279.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMarioOroshi
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListdimethylated residue
InstrumentBruker Daltonics timsTOF series; timsTOF SCP
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-12-08 16:38:03ID requested
12023-10-03 10:35:16announced
22023-11-14 07:37:35announced2023-11-14: Updated project metadata.
Publication List
Keyword List
submitter keyword: Single-cell proteomics, machine learning, hepatocytes, tissue heterogeneity, spatial omics, liver
Contact List
MatthiasMann
contact affiliationDepartment of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Martinsried, Germany
contact emailmmann@biochem.mpg.de
lab head
MarioOroshi
contact affiliationProteomics
contact emailoroshi@biochem.mpg.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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