PXD038695 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The ATAC and SAGA coactivator complexes assemble co-translationally, but their cellular localization properties and functions are distinct |
Description | To understand the precise mechanism that guide the formation of multisubunit complexes is of key importance. Nascent proteins can find and bind their interaction partners during their translation, leading to co-translational assembly. Here we demonstrate that the distinct modules of ATAC (ADA Two A Containing) and SAGA (SPT ADA GCN5 Acetyltransferase), two lysine acetyl transferase-containing transcription coactivator complexes, assemble co-translationally in the cytoplasm of mammalian cells. Fully assembled SAGA complex forms in the cytoplasm of mammalian cells and cytoplasmic SAGA acetylates non-histones proteins, before imported in the nucleus. In contrast, ATAC has no cytoplasmic functions as it cannot be detected in the cytoplasm of mammalian cells. However, fully assembled endogenous ATAC complex containing two functional modules forms and functions in the nucleus. Thus, the two related co-activators, ATAC and SAGA, assemble by using co-translational pathways, but their subcellular localization, cytoplasmic detectability and functions are distinct. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:25:28.462.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Luc Negroni |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-12-08 16:24:53 | ID requested | |
1 | 2023-09-27 05:29:41 | announced | |
⏵ 2 | 2023-11-14 08:25:28 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: SAGA,ATAC, subcellular localization, subcellular function |
Contact List
Laszlo Tora |
contact affiliation | IGBMC,Illkirch,France ORCID iD: 0000-0001-7398-2250 |
contact email | laszlo@igbmc.fr |
lab head | |
Luc Negroni |
contact affiliation | CNRS |
contact email | luc.negroni@igbmc.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD038695
- Label: PRIDE project
- Name: The ATAC and SAGA coactivator complexes assemble co-translationally, but their cellular localization properties and functions are distinct