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PXD038639-1

PXD038639 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleConservation of the PBL-RBOH immune module in land plants
DescriptionThe rapid production of reactive oxygen species (ROS) is a key signaling output in plant immunity. In the angiosperm model species, Arabidopsis thaliana (hereafter Arabidopsis), recognition of non- or altered-self elicitor patterns by cell-surface immune receptors activates the receptor-like cytoplasmic kinases (RLCKs) of the AVRPPHB SUSCEPTIBLE 1 (PBS1)-like (PBL) family, particularly BOTRYTIS-INDUCED KINASE1 (BIK1)​1–3​. BIK1/PBLs in turn phosphorylate the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase RESPIRATORY BURST OXIDASE HOMOLOG D (RBOHD) to induce apoplastic ROS production​4,5​. PBL and RBOH functions in plant immunity have been extensively characterized in flowering plants. Much less is known about the conservation of pattern-triggered ROS signaling pathways in non-flowering plants. In this study, we show that, in the liverwort Marchantia polymorpha (hereafter Marchantia), single members of RBOH and PBL families, namely MpRBOH1 and MpPBLa, are required for chitin-induced ROS production. MpPBLa directly interacts with and phosphorylates MpRBOH1 at specific, conserved sites within its cytosolic N-terminus and this phosphorylation is essential for chitin-induced MpRBOH1-mediated ROS production. Collectively, our work reveals the functional conservation of the PBL-RBOH module that controls pattern-triggered ROS production in land plants.
HostingRepositoryPRIDE
AnnounceDate2023-02-06
AnnouncementXMLSubmission_2023-02-06_03:21:00.412.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFRankMenke
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Marchantia polymorpha; NCBI TaxID: 3197;
ModificationListphosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-12-07 07:19:44ID requested
12023-02-06 03:21:00announced
22023-11-14 08:58:37announced2023-11-14: Updated project metadata.
Publication List
Keyword List
submitter keyword: Oxidative burst,Marchantia, kinase, Phosphorylation
Contact List
FrankMenke
contact affiliationThe Sainsbury Laboratory
contact emailfrank.menke@tsl.ac.uk
lab head
FRankMenke
contact affiliationthe Sainsbury laboratory
contact emailFrank.Menke@tsl.ac.uk
dataset submitter
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