PXD038639

PXD038639 is an original dataset announced via ProteomeXchange.

Title	Conservation of the PBL-RBOH immune module in land plants
Description	The rapid production of reactive oxygen species (ROS) is a key signaling output in plant immunity. In the angiosperm model species, Arabidopsis thaliana (hereafter Arabidopsis), recognition of non- or altered-self elicitor patterns by cell-surface immune receptors activates the receptor-like cytoplasmic kinases (RLCKs) of the AVRPPHB SUSCEPTIBLE 1 (PBS1)-like (PBL) family, particularly BOTRYTIS-INDUCED KINASE1 (BIK1)​1–3​. BIK1/PBLs in turn phosphorylate the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase RESPIRATORY BURST OXIDASE HOMOLOG D (RBOHD) to induce apoplastic ROS production​4,5​. PBL and RBOH functions in plant immunity have been extensively characterized in flowering plants. Much less is known about the conservation of pattern-triggered ROS signaling pathways in non-flowering plants. In this study, we show that, in the liverwort Marchantia polymorpha (hereafter Marchantia), single members of RBOH and PBL families, namely MpRBOH1 and MpPBLa, are required for chitin-induced ROS production. MpPBLa directly interacts with and phosphorylates MpRBOH1 at specific, conserved sites within its cytosolic N-terminus and this phosphorylation is essential for chitin-induced MpRBOH1-mediated ROS production. Collectively, our work reveals the functional conservation of the PBL-RBOH module that controls pattern-triggered ROS production in land plants.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:58:36.812.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	FRank Menke
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Marchantia polymorpha; NCBI TaxID: 3197;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-07 07:19:44	ID requested
1	2023-02-06 03:21:00	announced
⏵ 2	2023-11-14 08:58:37	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Oxidative burst,Marchantia, kinase, Phosphorylation

Frank Menke
contact affiliation	The Sainsbury Laboratory
contact email	frank.menke@tsl.ac.uk
lab head
FRank Menke
contact affiliation	the Sainsbury laboratory
contact email	Frank.Menke@tsl.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038639
     1. Label: PRIDE project
     2. Name: Conservation of the PBL-RBOH immune module in land plants