PXD038440 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Quantitative timecourse analysis of oncogenic KRAS expression impact on the proteme of pancreatic ductal adenocarcinoma cells. |
Description | To reveal the impact of mutant KRAS expression on the proteome of Pancreatic Ductal Adenocarcinoma (PDAC) cells, we carried out a timecourse quantitative proteomic analysis of tumour cells isolated from an inducible mouse model of PDAC (iKras PDAC) (Ying et al., 2012). In this model, oncogenic Kras (G12D) expression can be controlled by administration of doxycycline(Dox). Cells were grown in the absence of Dox for 48 hrs, before being treated with or without Dox for 4, 8, 12, 24, and 36 hrs, followed by total lysis and quantitative proteomics analysis using Tandem Mass Tagging (TMT). A total of 2 biological replicate experiments were analysed, with all samples from each replicate barcoded and pooled together using TMT 10plex labelling kit (Thermo). |
HostingRepository | PRIDE |
AnnounceDate | 2023-02-12 |
AnnouncementXML | Submission_2023-02-12_07:40:21.003.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | FarazMardakheh |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-11-27 01:30:43 | ID requested | |
⏵ 1 | 2023-02-12 07:40:21 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: TMT |
Quantitative proteomics |
KRAS |
PDAC |
Contact List
Faraz K.Mardakheh |
contact affiliation | Centre for Cancer Cell & Molecular Biology, Barts Cancer Institute, Queen Mary University of London, Charterhouse Square, London EC1M 6BQ, UK. |
contact email | f.mardakheh@qmul.ac.uk |
lab head | |
FarazMardakheh |
contact affiliation | Barts Cancer Institute |
contact email | f.mardakheh@qmul.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD038440
- Label: PRIDE project
- Name: Quantitative timecourse analysis of oncogenic KRAS expression impact on the proteme of pancreatic ductal adenocarcinoma cells.