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PXD038303-1

PXD038303 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleFucosylation of HLA-DRB1 regulates CD4+T cell-mediated anti-melanoma immunity and enhances immunotherapy efficacy: Fucose Click Chemistry
DescriptionImmunotherapy efficacy is limited in melanoma, and combinations of immunotherapies with other modalities have yielded limited improvements but also adverse events requiring cessation of treatment. In addition to ineffective patient stratification, efficacy is impaired by paucity of intratumoral immune cells (itICs)—thus, effective strategies to safely increase itICs are needed. We report that dietary administration of L-fucose induces fucosylation and cell surface enrichment of the MHC-II protein HLA-DRB1 in melanoma cells, triggering CD4+T cell-mediated increases in ItICs and anti-tumor immunity, enhancing immune checkpoint blockade responses. Melanoma fucosylation and fucosylated HLA-DRB1 associate with intratumoral T cell abundance and anti-PD1 responder status in patient melanoma specimens, suggesting the potential use of melanoma fucosylation as a strategy for stratifying patients for immunotherapies. Our findings demonstrate that fucosylation is a key mediator of anti-tumor immunity, and importantly, suggest that L-fucose is a powerful agent for safely increasing ItICs and immunotherapy efficacy in melanoma.
HostingRepositoryPRIDE
AnnounceDate2022-12-16
AnnouncementXMLSubmission_2022-12-15_19:14:14.321.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD038303
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterJohnKoomen
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-11-23 01:59:12ID requested
12022-12-15 19:14:14announced
Publication List
10.6019/PXD038303;
Keyword List
submitter keyword: Fucosylation,Melanoma, HLA-DRB1, Immunotherapy
Contact List
Eric Lau,PhD
contact affiliationTumor Biology Department Molecular Medicine Program Moffitt Cancer Center Tampa, FL, USA
contact emaileric.lau@moffitt.org
lab head
JohnKoomen
contact affiliationMoffitt Cancer Center
contact emailjohn.koomen@moffitt.org
dataset submitter
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Dataset FTP location
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