PXD038135 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Tartrate resistant acid phosphatase 5 (TRAP5) mediates immune cell recruitment in a murine model of pulmonary bacterial infection |
Description | During airway infection, upregulation of proinflammatory cytokines and subsequent immune cell recruitment is essential to mitigate bacterial infection. Conversely, during prolonged and non-resolving airway inflammation, neutrophils contribute to tissue damage and remodeling. This occurs during diseases including cystic fibrosis (CF) and COPD where bacterial pathogens, not least Pseudomonas aeruginosa, contribute to disease progression through long-lasting infections. Tartrate-resistant acid phosphatase (TRAP) 5 is a metalloenzyme expressed by alveolar macrophages and one of its target substrates is the phosphoglycoprotein osteopontin (OPN). In this study, we found that TRAP5-/- mice had impaired clearance of P. aeruginosa airway infection and reduced recruitment of immune cells. TRAP5 knockdown using siRNA resulted in a decreased activation of the proinflammatory transcription factor NF‐B in reporter mice and a subsequent decrease of proinflammatory gene expression. Add‐back experiments of enzymatically active TRAP5 to TRAP5-/- mice restored immune cell recruitment and bacterial killing. In human CF lung tissue, TRAP5 of alveolar macrophages was detected in proximity to OPN to a higher degree than in normal lung tissue, indicating possible interactions. Taken together, the findings of this study suggest a key role for TRAP5 in modulating airway inflammation. This could have bearing in diseases such as CF and COPD where excessive sterile inflammation could be targeted by pharmacological inhibitors of TRAP5. |
HostingRepository | PRIDE |
AnnounceDate | 2022-12-30 |
AnnouncementXML | Submission_2022-12-30_07:22:41.870.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | CharlotteWelinder |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue; monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-11-14 03:11:03 | ID requested | |
⏵ 1 | 2022-12-30 07:22:42 | announced | |
2 | 2023-11-14 07:42:34 | announced | 2023-11-14: Updated project metadata. |
Publication List
Tanner L, Bergwik J, Bhongir RKV, Puthia M, L, å, ng P, Ali MN, Welinder C, Ö, nnerfjord P, Erjef, ä, lt JS, Palmberg L, Andersson G, Egesten A, Tartrate resistant acid phosphatase 5 (TRAP5) mediates immune cell recruitment in a murine model of pulmonary bacterial infection. Front Immunol, 13():1079775(2022) [pubmed] |
Keyword List
submitter keyword: NF‐B, COPD, ACP5, SPP1,TRAP5, Pseudomonas aeruginosa, Cystic Fibrosis |
Contact List
ArneEgesten |
contact affiliation | Department of Respiratory Medicine and Allergology, Faculty of Medicine, Lund University, Sweden |
contact email | arne.egesten@med.lu.se |
lab head | |
CharlotteWelinder |
contact affiliation | Dept. of Oncology, Clinical Sciences, Lund University, 221 85 Lund, Sweden |
contact email | charlotte.welinder@med.lu.se |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/12/PXD038135 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD038135
- Label: PRIDE project
- Name: Tartrate resistant acid phosphatase 5 (TRAP5) mediates immune cell recruitment in a murine model of pulmonary bacterial infection