PXD038135

PXD038135 is an original dataset announced via ProteomeXchange.

Title	Tartrate resistant acid phosphatase 5 (TRAP5) mediates immune cell recruitment in a murine model of pulmonary bacterial infection
Description	During airway infection, upregulation of proinflammatory cytokines and subsequent immune cell recruitment is essential to mitigate bacterial infection. Conversely, during prolonged and non-resolving airway inflammation, neutrophils contribute to tissue damage and remodeling. This occurs during diseases including cystic fibrosis (CF) and COPD where bacterial pathogens, not least Pseudomonas aeruginosa, contribute to disease progression through long-lasting infections. Tartrate-resistant acid phosphatase (TRAP) 5 is a metalloenzyme expressed by alveolar macrophages and one of its target substrates is the phosphoglycoprotein osteopontin (OPN). In this study, we found that TRAP5-/- mice had impaired clearance of P. aeruginosa airway infection and reduced recruitment of immune cells. TRAP5 knockdown using siRNA resulted in a decreased activation of the proinflammatory transcription factor NF‐B in reporter mice and a subsequent decrease of proinflammatory gene expression. Add‐back experiments of enzymatically active TRAP5 to TRAP5-/- mice restored immune cell recruitment and bacterial killing. In human CF lung tissue, TRAP5 of alveolar macrophages was detected in proximity to OPN to a higher degree than in normal lung tissue, indicating possible interactions. Taken together, the findings of this study suggest a key role for TRAP5 in modulating airway inflammation. This could have bearing in diseases such as CF and COPD where excessive sterile inflammation could be targeted by pharmacological inhibitors of TRAP5.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:42:33.328.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Charlotte Welinder
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-14 03:11:03	ID requested
1	2022-12-30 07:22:42	announced
⏵ 2	2023-11-14 07:42:34	announced	2023-11-14: Updated project metadata.

Tanner L, Bergwik J, Bhongir RKV, Puthia M, L, å, ng P, Ali MN, Welinder C, Ö, nnerfjord P, Erjef, ä, lt JS, Palmberg L, Andersson G, Egesten A, Tartrate resistant acid phosphatase 5 (TRAP5) mediates immune cell recruitment in a murine model of pulmonary bacterial infection. Front Immunol, 13():1079775(2022) [pubmed]

submitter keyword: NF‐B, COPD, ACP5, SPP1,TRAP5, Pseudomonas aeruginosa, Cystic Fibrosis

Arne Egesten
contact affiliation	Department of Respiratory Medicine and Allergology, Faculty of Medicine, Lund University, Sweden
contact email	arne.egesten@med.lu.se
lab head
Charlotte Welinder
contact affiliation	Dept. of Oncology, Clinical Sciences, Lund University, 221 85 Lund, Sweden
contact email	charlotte.welinder@med.lu.se
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038135
     1. Label: PRIDE project
     2. Name: Tartrate resistant acid phosphatase 5 (TRAP5) mediates immune cell recruitment in a murine model of pulmonary bacterial infection