PXD038114

PXD038114 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometry imaging of ancient teeth proteomes
Description	In order to research the variation in protein distribution in teeth, proteins were extracted from archaeological (15-18th century, Netherlands) and modern teeth and identified using LC-MS/MS. Of the recovered proteins we then visualised the distribution of collagen type I (both the alpha-1 and -2 chains), alpha-2-HS-glycoprotein, haemoglobin subunit alpha and myosin light polypeptide 6 using MALDI-MSI. We found distinct differences in the spatial distributions of different proteins as well as between some peptides of the same protein. The reason for these differences in protein spatial distribution remain unclear, yet this study highlights the ability of MALDI-MSI for visualisng the spatial distribution of proteins in archaeological biomineralised tissues. Therefore MALDI-MSI might prove a useful tool to improve our understanding of protein preservation as well as aid in deciding sampling strategies.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:43:49.679.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD038114
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jan Dekker
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; SYNAPT G2-Si

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-11 09:30:01	ID requested
1	2023-03-11 11:07:40	announced
⏵ 2	2023-11-14 08:43:50	announced	2023-11-14: Updated project metadata.

Dekker J, Larson T, Tzvetkov J, Harvey VL, Dowle A, Hagan R, Genever P, Schrader S, Soressi M, Hendy J, Spatial analysis of the ancient proteome of archeological teeth using mass spectrometry imaging. Rapid Commun Mass Spectrom, 37(8):e9486(2023) [pubmed]

10.6019/PXD038114;

submitter keyword: palaeoproteomics, teeth, protein distribution,MALDI-MSI, diagenesis

Jessica Hendy
contact affiliation	BioArCh, Department of Archaeology, University of York
contact email	jessica.hendy@york.ac.uk
lab head
Jan Dekker
contact affiliation	University of York
contact email	jan@palaeome.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD038114

PRIDE project URI

• PRIDE
  1. PXD038114
     1. Label: PRIDE project
     2. Name: Mass spectrometry imaging of ancient teeth proteomes