PXD038039 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Insights into the sulfur metabolism of Chlorobaculum tepidum by label free quantitative proteomics |
Description | Chlorobaculum tepidum is an anaerobic green sulfur bacterium which oxidizes sulfide, elemental sulfur, and thiosulfate for photosynthetic growth. It can also oxidize sulfide to produce extracellular S0 globules, which can be further oxidized to sulfate and used as an electron donor. Here we performed label free quantitative proteomics on total cell lysates prepared from different metabolic states, including a sulfur production state (10 hours post incubation, PI), the beginning of sulfur consumption (20 hours PI) and the end of sulfur consumption (40 hours PI), respectively. We observed an increased abundance of the sulfide:quinone oxidoreductase Sqr proteins in 20PI indicated a sulfur production state. The periplasmic thiosulfate-oxidizing Sox enzymes and the dissimilatory sulfite reductase Dsr subunits showed an increased abundance in 20PI, corresponding to the sulfur-consuming state. In addition, we found that the abundance of the heterodisulfide-reductase and the sulfhydrogenase operons was influenced of electron donor availability and may be associated with sulfur metabolism. Further, we isolated and analyzed the extracellular sulfur globules in the different metabolic states in order to study their morphology and the sulfur cluster composition, yielding 58 previously uncharacterized proteins in purified globules. Our results show that Cba tepidum regulates the cellular levels of enzymes involved in sulfur metabolism in response to the availability of reduced sulfur compounds. |
HostingRepository | PRIDE |
AnnounceDate | 2023-02-13 |
AnnouncementXML | Submission_2023-02-13_06:52:23.473.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | JulianLanger |
SpeciesList | scientific name: Chlorobaculum tepidum TLS; NCBI TaxID: 194439; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos; rapifleX |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-11-08 07:38:04 | ID requested | |
⏵ 1 | 2023-02-13 06:52:23 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: "sulfur metabolism", LFQ,"Chlorobaculum tepidum" |
Contact List
Julian D.Langer |
contact affiliation | Max Planck Institute of Biophysics Max Planck Institute for Brain Research |
contact email | julian.langer@biophys.mpg.de |
lab head | |
JulianLanger |
contact affiliation | MPIs for Biophysics and Brain Research |
contact email | julian.langer@biophys.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD038039
- Label: PRIDE project
- Name: Insights into the sulfur metabolism of Chlorobaculum tepidum by label free quantitative proteomics