PXD038039

PXD038039 is an original dataset announced via ProteomeXchange.

Title	Insights into the sulfur metabolism of Chlorobaculum tepidum by label free quantitative proteomics
Description	Chlorobaculum tepidum is an anaerobic green sulfur bacterium which oxidizes sulfide, elemental sulfur, and thiosulfate for photosynthetic growth. It can also oxidize sulfide to produce extracellular S0 globules, which can be further oxidized to sulfate and used as an electron donor. Here we performed label free quantitative proteomics on total cell lysates prepared from different metabolic states, including a sulfur production state (10 hours post incubation, PI), the beginning of sulfur consumption (20 hours PI) and the end of sulfur consumption (40 hours PI), respectively. We observed an increased abundance of the sulfide:quinone oxidoreductase Sqr proteins in 20PI indicated a sulfur production state. The periplasmic thiosulfate-oxidizing Sox enzymes and the dissimilatory sulfite reductase Dsr subunits showed an increased abundance in 20PI, corresponding to the sulfur-consuming state. In addition, we found that the abundance of the heterodisulfide-reductase and the sulfhydrogenase operons was influenced of electron donor availability and may be associated with sulfur metabolism. Further, we isolated and analyzed the extracellular sulfur globules in the different metabolic states in order to study their morphology and the sulfur cluster composition, yielding 58 previously uncharacterized proteins in purified globules. Our results show that Cba tepidum regulates the cellular levels of enzymes involved in sulfur metabolism in response to the availability of reduced sulfur compounds.
HostingRepository	PRIDE
AnnounceDate	2023-02-13
AnnouncementXML	Submission_2023-02-13_06:52:23.473.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	JulianLanger
SpeciesList	scientific name: Chlorobaculum tepidum TLS; NCBI TaxID: 194439;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; rapifleX

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-08 07:38:04	ID requested
⏵ 1	2023-02-13 06:52:23	announced

Dataset with its publication pending

submitter keyword: "sulfur metabolism", LFQ,"Chlorobaculum tepidum"

Julian D.Langer
contact affiliation	Max Planck Institute of Biophysics Max Planck Institute for Brain Research
contact email	julian.langer@biophys.mpg.de
lab head
JulianLanger
contact affiliation	MPIs for Biophysics and Brain Research
contact email	julian.langer@biophys.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/02/PXD038039

PRIDE project URI

• PRIDE
  1. PXD038039
     1. Label: PRIDE project
     2. Name: Insights into the sulfur metabolism of Chlorobaculum tepidum by label free quantitative proteomics