<<< Full experiment listing

PXD038038-1

PXD038038 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRevealing Corynebacterium glutamicum proteoforms through top-down proteomics
DescriptionCorynebacterium glutamicum is a bacterium widely employed in the industrial production of amino acids as well as a broad range of other biotechnological products. The present study describes the characterization of C. glutamicum proteoforms, and their post-translational modifications (PTMs) employing top-down proteomics. Despite previous evidence of PTMs having roles in the regulation of C. glutamicum metabolism, this is the first top-down proteome analysis of this organism. We identified 1125 proteoforms from 273 proteins, with 60 % of proteins presenting at least one mass shift, suggesting the presence of PTMs, including several acetylated, oxidized and formylated proteoforms. Furthermore, proteins relevant to amino acid production, protein secretion, and oxidative stress were identified with mass shifts suggesting the presence of uncharacterized PTMs and proteoforms that may affect biotechnologically relevant processes in this industrial workhorse. For instance, the membrane proteins mepB and SecG were identified as a cleaved and a formylated proteoform, respectively. While in the central metabolism, OdhI was identified as two proteoforms with potential biological relevance: a cleaved proteoform and a proteoform with PTMs corresponding to a 70 Da mass shift.
HostingRepositoryPRIDE
AnnounceDate2022-12-10
AnnouncementXMLSubmission_2022-12-10_05:13:13.536.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterReynaldoMagalhaes Melo
SpeciesList scientific name: Corynebacterium glutamicum ATCC 13032; NCBI TaxID: 196627;
ModificationListacetylated residue
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-11-08 07:37:43ID requested
12022-12-10 05:13:13announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: top-down proteomics, Corynebacterium glutamicum,Proteoforms
Contact List
Marcelo Valle deSousa
contact affiliationLaboratory of Protein Chemistry and Biochemistry, Department of Cell Biology, Institute of Biology, University of Brasilia, Brasilia, Brazil.
contact emailmvsousa@unb.br
lab head
ReynaldoMagalhaes Melo
contact affiliationUniversity of Brasilia
contact emailreynaldommelo@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/12/PXD038038
PRIDE project URI
Repository Record List
[ + ]