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PXD038027-1

PXD038027 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProtocol for cell surface biotinylation of magnetic labeled and captured human peripheral blood mononuclear cells
DescriptionAnalysis of the surfaceome of a blood cell subset requires cell sorting, followed by surface protein enrichment. Here, we present a protocol combining magnetically activated cell sorting (MACS) and surface biotinylation of the target cell subset from human peripheral blood mononuclear cells (PBMCs). We describe the steps for isolating target cells and their in-column surface biotinylation, followed by isolation and mass spectrometry analysis of biotinylated proteins. The protocol enables in-column surface biotinylation of specific cell subsets with minimal membrane disruption.
HostingRepositoryPRIDE
AnnounceDate2022-11-08
AnnouncementXMLSubmission_2022-11-08_01:56:27.267.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterServetÖZCAN
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListbiotinylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-11-07 10:19:59ID requested
12022-11-08 01:56:27announced
22023-11-14 08:59:21announced2023-11-14: Updated project metadata.
Publication List
Keyword List
submitter keyword: MACS, surface proteome, biotinylation
Contact List
ServetÖZCAN
contact affiliationFaculty of Science, Department of Biology, Erciyes University, Kayseri 38039, Türkiye Genome and Stem Cell Center (GENKÖK), Erciyes University, Kayseri 38039, Türkiye
contact emailozcan@erciyes.edu.tr
lab head
ServetÖZCAN
contact affiliationErciyes University Genkök
contact emailozcan@erciyes.edu.tr
dataset submitter
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