PXD038000-2
PXD038000 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Moonlighting chaperone 1 activity of the enzyme PqsE contributes to RhlR-controlled virulence of Pseudomonas aeruginosa |
Description | Pseudomonas aeruginosa is a major cause of nosocomial infections and also leads to severe exacerbations in cystic fibrosis or chronic obstructive pulmonary disease. Three intertwined quorum sensing systems control virulence of P. aeruginosa, with the rhl circuit playing the leading role in late and chronic infections. The majority of traits controlled by rhl transcription factor RhlR depend on PqsE, a dispensable thioesterase in Pseudomonas Quinolone Signal (PQS) biosynthesis that interferes with RhlR through an enigmatic mechanism likely involving direct interaction of both proteins. Here we show that PqsE and RhlR form a 2:2 protein complex that, together with RhlR agonist N-butanoyl-L-homoserine lactone (C4-HSL), solubilizes RhlR and thereby renders the otherwise insoluble transcription factor active. We determined crystal structures of the complex and identified residues essential for the interaction. To corroborate the chaperone like activity of PqsE, we designed stability-optimized variants of RhlR that bypass the need for C4-HSL and PqsE in activating PqsE/RhlR-controlled processes of P. aeruginosa. Together, our data provide insight into the unique regulatory role of PqsE and lay groundwork for developing new P. aeruginosa-specific pharmaceuticals. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:42:45.052.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Lothar Jaensch |
SpeciesList | scientific name: Pseudomonas aeruginosa PA14; NCBI TaxID: 652611; |
ModificationList | iodoacetamide derivatized residue |
Instrument | timsTOF Pro; LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2022-11-07 09:43:14 | ID requested | |
1 | 2023-03-11 04:03:19 | announced | |
⏵ 2 | 2023-11-14 08:42:53 | announced | 2023-11-14: Updated project metadata. |
Publication List
Borgert SR, Henke S, Witzgall F, Schmelz S, Zur Lage S, Hotop SK, Stephen S, L, ü, bken D, Kr, ü, ger J, Gomez NO, van Ham M, J, ä, nsch L, Kalesse M, Pich A, Br, ö, nstrup M, H, ä, ussler S, Blankenfeldt W, Moonlighting chaperone activity of the enzyme PqsE contributes to RhlR-controlled virulence of Pseudomonas aeruginosa. Nat Commun, 13(1):7402(2022) [pubmed] |
Keyword List
submitter keyword: RhlR,Pseudomonas aeruginosa, PqsE, quorum sensing, LC-MS/MS |
Contact List
Lothar Jänsch, Wulf Blankenfeldt | |
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contact affiliation | Department Structure and Function of Proteins Helmholtz Centre for Infection Research Inhoffenstr. 7, 38124 Braunschweig, Germany |
contact email | lothar.jaensch@helmholtz-hzi.de |
lab head | |
Lothar Jaensch | |
contact affiliation | HZI |
contact email | Lothar.Jaensch@Helmholtz-HZI.de |
dataset submitter |
Full Dataset Link List
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PRIDE project URI |
Repository Record List
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