PXD037966-2

PXD037966 is an original dataset announced via ProteomeXchange.

Title	Hypoxic preconditioned neural stem cell derived extracellular vesiclescontain distinct protein cargo from their normal counterparts
Description	Hypoxic pre-conditioning has been demonstrated to increase the resistance of neural stem cells (NSCs) to hypoxic conditions, as well as to improve their capacity for differentiation and neuro-genesis. Extracellular vesicles (EVs) have recently emerged as critical mediators of cell-cell com-munication, but their role in this hypoxic conditioning is presently unknown. Here, we demon-strated that three hours of hypoxic pre-conditioning triggers significant neural stem cell EV release. Proteomic profiling of EVs from normal and hypoxic preconditioned neural stem cells identified 20 proteins that were up-regulated and 22 proteins that were down-regulated after hypoxic precon-ditioning. We also found an upregulation of some of these proteins by qPCR, thus indicating dif-ferences also at the transcript level within the EVs. Among the up-regulated proteins are CNP, Cyfip1, CASK, and TUBB5, which are well known to exhibit significant beneficial effects to neural stem cells. Thus, our results not only show a significant difference of protein cargo in EVs conse-quent to hypoxic exposure, but identify several candidate proteins that might play a pivotal role in the cell-to-cell mediated communication underlying neuronal differentiation, protection, matura-tion and survival following exposure to hypoxic conditions.
HostingRepository	iProX
AnnounceDate	2022-11-07
AnnouncementXML	Submission_2023-04-03_00:37:23.729.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tahereh Gharbi
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-06 23:46:03	ID requested
1	2022-11-06 23:50:47	announced
⏵ 2	2023-04-03 00:37:24	announced	2023-04-03: Update publication information.

Gharbi T, Liu C, Khan H, Zhang Z, Yang GY, Tang Y, Hypoxic Preconditioned Neural Stem Cell-Derived Extracellular Vesicles Contain Distinct Protein Cargo from Their Normal Counterparts. Curr Issues Mol Biol, 45(3):1982-1997(2023) [pubmed]

submitter keyword: mouse neural stem cell derived extracellular vesicles,iTRAQ

Yaohui Tang
contact affiliation	Shanghai Jiao Tong University
contact email	yaohuitang@sjtu.edu.cn
lab head
Tahereh Gharbi
contact affiliation	Shanghai Jiao Tong University
contact email	gharbitahereh@yahoo.com
dataset submitter

iProX dataset URI