PXD037966
PXD037966 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Hypoxic preconditioned neural stem cell derived extracellular vesiclescontain distinct protein cargo from their normal counterparts |
Description | Hypoxic pre-conditioning has been demonstrated to increase the resistance of neural stem cells (NSCs) to hypoxic conditions, as well as to improve their capacity for differentiation and neuro-genesis. Extracellular vesicles (EVs) have recently emerged as critical mediators of cell-cell com-munication, but their role in this hypoxic conditioning is presently unknown. Here, we demon-strated that three hours of hypoxic pre-conditioning triggers significant neural stem cell EV release. Proteomic profiling of EVs from normal and hypoxic preconditioned neural stem cells identified 20 proteins that were up-regulated and 22 proteins that were down-regulated after hypoxic precon-ditioning. We also found an upregulation of some of these proteins by qPCR, thus indicating dif-ferences also at the transcript level within the EVs. Among the up-regulated proteins are CNP, Cyfip1, CASK, and TUBB5, which are well known to exhibit significant beneficial effects to neural stem cells. Thus, our results not only show a significant difference of protein cargo in EVs conse-quent to hypoxic exposure, but identify several candidate proteins that might play a pivotal role in the cell-to-cell mediated communication underlying neuronal differentiation, protection, matura-tion and survival following exposure to hypoxic conditions. |
HostingRepository | iProX |
AnnounceDate | 2022-11-07 |
AnnouncementXML | Submission_2023-04-03_00:37:23.729.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Tahereh Gharbi |
SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2022-11-06 23:46:03 | ID requested | |
1 | 2022-11-06 23:50:47 | announced | |
⏵ 2 | 2023-04-03 00:37:24 | announced | 2023-04-03: Update publication information. |
Publication List
Gharbi T, Liu C, Khan H, Zhang Z, Yang GY, Tang Y, Hypoxic Preconditioned Neural Stem Cell-Derived Extracellular Vesicles Contain Distinct Protein Cargo from Their Normal Counterparts. Curr Issues Mol Biol, 45(3):1982-1997(2023) [pubmed] |
Keyword List
submitter keyword: mouse neural stem cell derived extracellular vesicles,iTRAQ |
Contact List
Yaohui Tang | |
---|---|
contact affiliation | Shanghai Jiao Tong University |
contact email | yaohuitang@sjtu.edu.cn |
lab head | |
Tahereh Gharbi | |
contact affiliation | Shanghai Jiao Tong University |
contact email | gharbitahereh@yahoo.com |
dataset submitter |
Full Dataset Link List
iProX dataset URI |