PXD037910-1

PXD037910 is an original dataset announced via ProteomeXchange.

Title	Mouse osteoclast homogenates nLC-ESI-MS/MS - WT vs Slc37a2 KO
Description	To survey the proteomic differences between WT and Slc37a2 knockout osteoclasts at the whole cell and secretory lysosome levels, we used superparamagnetic iron oxide nanoparticles (SPIONs) to enrich for these endo-lysosomal-related organelles from murine osteoclast cultures. Briefly, large scale murine bone marrow monocyte (BMM)-derived osteoclast cultures were ‘pulsed’ with SPIONs to encourage uptake into endosomes and then ‘chased’ into secretory lysosomes upon the convergence of SPION-loaded endosomes with lysosomes and secretory pathways. Following the ‘pulse-chase’, osteoclasts were homogenized, SPION-loaded organelles captured-from post-nuclear supernatants using magnetic columns, and enriched organelles as well as homogenates eluted and processed for 1D in-gel digestion and mass spectrometry. The samples presented here correspond to the proteome of WT and Slc37a2 knockout homogenates with secretory lysosome proteomes have been shared in another submission.
HostingRepository	PRIDE
AnnounceDate	2023-01-25
AnnouncementXML	Submission_2023-01-24_19:45:18.869.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	AmyRibet
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-03 04:22:40	ID requested
⏵ 1	2023-01-24 19:45:19	announced

Dataset with its publication pending

submitter keyword: Lysosome, LRO, Mouse, SPION,Osteoclast, Secretory lysosome

NathanPavlos
contact affiliation	The Bone Biology and Disease Laboratory, School of Biomedical Sciences, The University of Western Australia
contact email	nathan.pavlos@uwa.edu.au
lab head
AmyRibet
contact affiliation	The University of Western Australia
contact email	21703693@student.uwa.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/01/PXD037910

PRIDE project URI

• PRIDE
  1. PXD037910
     1. Label: PRIDE project
     2. Name: Mouse osteoclast homogenates nLC-ESI-MS/MS - WT vs Slc37a2 KO