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PXD037906-1

PXD037906 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHuman Tim8a, Tim8b and Tim13 are auxiliary assembly factors of mature Complex IV
DescriptionHuman Tim8a and Tim8b are paralogous intermembrane space proteins of the small TIM chaperone family. Yeast small TIMs function in the trafficking of proteins to the outer and inner mitochondrial membranes. This putative import function for hTim8a and hTim8b has been challenged in human models, but their true molecular function in cells has not been defined. Likewise, why human cells possess two Tim8 proteins and any potential redundancy is not clear. We demonstrate that hTim8a and hTim8b function in the assembly of cytochrome c oxidase (Complex IV). Using affinity enrichment mass spectrometry, we catalogue the interaction network of hTim8a, hTim8b and hTim13, identifying subunits and assembly factors of the Complex IV COX2 module. hTim8-deficient cells have a COX2/COX3 module defect, albeit less severe than a COX17KO cell line, and exhibit an accumulation of the S2 subcomplex. This suggests that hTim8a and hTim8b are required for assembly of Complex IV via interactions with intermembrane-space exposed subunits. We propose hTim8-hTim13 complexes act as chaperones to stabilise the intermediate S3 subcomplex, promoting assembly of monomeric Complex IV.
HostingRepositoryPRIDE
AnnounceDate2023-05-10
AnnouncementXMLSubmission_2023-05-09_22:34:54.803.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAlexAnderson
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Eclipse; Orbitrap Fusion Lumos; Q Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-11-03 04:19:10ID requested
12023-05-09 22:34:55announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: small TIMs, protein trafficking, mitochondria, protein assembly,Complex IV
Contact List
DianaStojanovski
contact affiliationDepartment of Biochemistry and Pharmacology, School of Biomedical Sciences, The University of Melbourne, Australia
contact emaild.stojanovski@unimelb.edu.au
lab head
AlexAnderson
contact affiliationUniversity of Melbourne
contact emailaanderson2@student.unimelb.edu.au
dataset submitter
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Dataset FTP location
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