PXD037906

PXD037906 is an original dataset announced via ProteomeXchange.

Title	Human Tim8a, Tim8b and Tim13 are auxiliary assembly factors of mature Complex IV
Description	Human Tim8a and Tim8b are paralogous intermembrane space proteins of the small TIM chaperone family. Yeast small TIMs function in the trafficking of proteins to the outer and inner mitochondrial membranes. This putative import function for hTim8a and hTim8b has been challenged in human models, but their true molecular function in cells has not been defined. Likewise, why human cells possess two Tim8 proteins and any potential redundancy is not clear. We demonstrate that hTim8a and hTim8b function in the assembly of cytochrome c oxidase (Complex IV). Using affinity enrichment mass spectrometry, we catalogue the interaction network of hTim8a, hTim8b and hTim13, identifying subunits and assembly factors of the Complex IV COX2 module. hTim8-deficient cells have a COX2/COX3 module defect, albeit less severe than a COX17KO cell line, and exhibit an accumulation of the S2 subcomplex. This suggests that hTim8a and hTim8b are required for assembly of Complex IV via interactions with intermembrane-space exposed subunits. We propose hTim8-hTim13 complexes act as chaperones to stabilise the intermediate S3 subcomplex, promoting assembly of monomeric Complex IV.
HostingRepository	PRIDE
AnnounceDate	2023-05-10
AnnouncementXML	Submission_2023-05-09_22:34:54.803.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	AlexAnderson
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse; Orbitrap Fusion Lumos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-03 04:19:10	ID requested
⏵ 1	2023-05-09 22:34:55	announced

Dataset with its publication pending

submitter keyword: small TIMs, protein trafficking, mitochondria, protein assembly,Complex IV

DianaStojanovski
contact affiliation	Department of Biochemistry and Pharmacology, School of Biomedical Sciences, The University of Melbourne, Australia
contact email	d.stojanovski@unimelb.edu.au
lab head
AlexAnderson
contact affiliation	University of Melbourne
contact email	aanderson2@student.unimelb.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD037906

PRIDE project URI

• PRIDE
  1. PXD037906
     1. Label: PRIDE project
     2. Name: Human Tim8a, Tim8b and Tim13 are auxiliary assembly factors of mature Complex IV