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PXD037795-1

PXD037795 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleExtracellular vesicles from Trypanosoma cruzi-dendritic cell interaction show modulatory properties and confer resistance to lethal infection as a cell-free based therapy strategy
DescriptionExtracellular vesicles (EVs) include a heterogeneous group of particles. Microvesicles and exosomes are the most characterised vesicles. They can be distinguished by their size, morphology, origin and molecular composition. To date, increasing studies demonstrate that EVs mediates intercellular communication. EVs reach considerable interest in the scientific community due to their role in diverse processes including antigen-presentation, stimulation of anti-tumoral immune responses, tolerogenic or inflammatory effects. In pathogens, EVs shedding is well described in fungus, bacteria, protozoan parasites and helminths. For Trypanosoma cruzi EVs liberation and protein composition was previously described. Dendritic cells (DCs) are key players promoting the immune response against pathogens and also maintaining self-tolerance. In previous reports we have demonstrate that T. cruzi downregulates DCs immunogenicity in vitro and in vivo. Here we analyze EVs from the in vitro interaction between blood circulating tripomastigotes (Tp) and bone-marrow-derived DCs. We found that Tp incremented the number and the size of EVs in cultures with DCs. EVs displayed some exosome markers and intracellular RNA. Protein analysis demonstrated that the parasite changes the protein-EV profile of DCs. We observed that EVs from the interaction of Tp-DCs were easily captured by unstimulated-DCs in comparison with EVs from DCs cultured without the parasite, and also modify the activation status of LPS-stimulated DCs. Noteworthy, we found protection in animal treated with EVs DCs+Tp and challenged with T. cruzi lethal infection. Our goal is to go deep into the molecular characterization of EVs from the DCs-Tp interaction, in order to identify mediators for therapeutic purposes.
HostingRepositoryPRIDE
AnnounceDate2022-11-09
AnnouncementXMLSubmission_2022-11-10_14:18:00.077.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLuzValero
SpeciesList scientific name: Trypanosoma cruzi; NCBI TaxID: 5693;
ModificationListNo PTMs are included in the dataset
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-10-28 07:13:00ID requested
12022-11-10 14:18:00announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: T. cruzi, Cell free therapy, Dendritic Cells, Extracellular vesicles (EVs), Immunotherapy
Contact List
Carolina V.Poncini
contact affiliationConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina
contact emailcvponcini@gmail.com
lab head
LuzValero
contact affiliationUniversitat de Valencia
contact emailmluz.valero@uv.es
dataset submitter
Full Dataset Link List
Dataset FTP location
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